机构：[1]Department of Urology, The First People’s Hospital of Yunnan Province, Kunming University of Science and Technology, Kunming, Yunnan Province, P. R. China外科片泌尿外科云南省第一人民医院
Recently, estrogen receptor β (ERβ) appears to be anti-proliferative and pro-apoptotic in normal prostate gland, but its role in androgen independent prostate cancer is limited. In this study, the expression of ERβ was overexpressed in two androgen-independent prostate cancer cell lines, PC-3 and DU145 after transfection with Ad- ERβ-EGFP virus particles. Overexpressed ERβ significantly inhibited cell proliferation in these two prostate cancer cell lines using MTT assay. Flow cytometry and Annexin V-APC/7-AAD double staining confirmed that upregulation of ERβ increased cell apoptotic rate. We found that upregulation of ERβ suppressed the expression of TGF-β1/IGF-1 expression, which could be reversed by ERβ-selective antagonist PHTPP. Consistently, TGF-β1 inhibitor LY2109761 treatment could weaken the effects of ERβ-selective antagonist PHTPP on the expression of IGF-1, survivin and bcl-2 in prostate cancer cells. In conclusion, these results suggest that estrogen may play an important role in androgen-independent prostate cancer cell proliferation through ERβ-mediated suppression of TGF-β1/IGF-1.
基金：
Kunming University
of Science and Technology Foundation (Grant
No.: kksy201460021).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|4 区医学
小类|4 区肿瘤学4 区病理学
最新[2025]版：
大类|4 区医学
小类|4 区肿瘤学4 区病理学
第一作者：
第一作者机构：[1]Department of Urology, The First People’s Hospital of Yunnan Province, Kunming University of Science and Technology, Kunming, Yunnan Province, P. R. China
通讯作者：
通讯机构：[1]Department of Urology, The First People’s Hospital of Yunnan Province, Kunming University of Science and Technology, Kunming, Yunnan Province, P. R. China[*1]Department of Urology, The First People’s Hospital of Yunnan Province, Kunming University of Science and Technology, 157 Jinbi Alley, Kunming 650041, Yunnan Province, P. R. China
推荐引用方式(GB/T 7714)：
Xiao L,Xiao M,Zou M,et al.Estrogen receptor β inhibits prostate cancer cell proliferation through downregulating TGF-β1/IGF-1 signaling[J].International Journal of Clinical and Experimental Pathology.2017,10(8):8569-8576.
APA：
Xiao, L,Xiao, M,Zou, M&Xu, W.(2017).Estrogen receptor β inhibits prostate cancer cell proliferation through downregulating TGF-β1/IGF-1 signaling.International Journal of Clinical and Experimental Pathology,10,(8)
MLA：
Xiao, L,et al."Estrogen receptor β inhibits prostate cancer cell proliferation through downregulating TGF-β1/IGF-1 signaling".International Journal of Clinical and Experimental Pathology 10..8(2017):8569-8576
