机构：[1]Department of Rheumatology, Nantong Hospital Affiliated to Nanjing, University of Traditional Chinese Medicine, Nantong, Jiangsu 226001, P. R. China[2]The No. 1 Affiliated Hospital of Yunnan, University of Traditional Chinese Medicine, Kunming 650021, P. R. China[3]Program in Cellular and Molecular Medicine, Boston Children’s Hospital and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Dioscin, a steroidal saponin isolated from Dioscorea nipponica Makino, has previously been shown to possess antiarthritic effects. However, the underlying mechanism is still elusive. Herein, we investigated the therapeutic effects of dioscin on collagen-induced arthritis (CIA) in DBA/1 mice and related mechanism. Cytokine production in CII-specific immune responses were measured by enzyme-linked immunosorbent assay (ELISA); Th17 cell-related gene expression, including IL-17A, ROR
γ
τ
and IL-23p19, were detected by qPCR analysis; Surface marker, T regulatory (Treg) cells and intracellular cytokines (IL-17A and IFN-
γ
) were evaluated by flow cytometry. We performed Th17 cell differentiation assay in vitro. Results showed that, in vivo, dioscin treatment significantly reduced the severity of CIA, which was accompanied by decreased Th17 response, but not Th1 and Treg response; dioscin-treated mice also showed lower percentage of CD11b
+
Gr-1
+
neutrophils; In vitro, dioscin treatment suppressed the differentiation of naive CD4
+
T cells into Th17 cell and decreased IL-17A production. Collectively, our results indicate that dioscin exerts antiarthritic effects by inhibiting Th17 cell immune response.
基金：
This work was financially supported by the Natural Science Foundation of China (grants
81460624 and 81360662), the Clinical Trial for the Treatment of Rheumatoid Arthritis
with Warming yang and Smoothening Meridians (grant 201507001-07), the Grants-in-Aid for Scientific Research (MS12017020-5) from the Ministry of Nantong Science and
Technology, the Applied Basic Research Program of Yunnan Province (grants 2015FB199
and 2016FA037) and the Joint Project from Yunnan Science and Technology Office and
Yunnan University (grant 2018FY001-001).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|3 区医学
小类|2 区全科医学与补充医学3 区医学：内科
最新[2023]版：
大类|2 区医学
小类|2 区全科医学与补充医学2 区医学：内科
第一作者：
第一作者机构：[1]Department of Rheumatology, Nantong Hospital Affiliated to Nanjing, University of Traditional Chinese Medicine, Nantong, Jiangsu 226001, P. R. China
共同第一作者：
通讯作者：
通讯机构：[2]The No. 1 Affiliated Hospital of Yunnan, University of Traditional Chinese Medicine, Kunming 650021, P. R. China[3]Program in Cellular and Molecular Medicine, Boston Children’s Hospital and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA[*1]Program in Cellular and Molecular Medicine, Boston Children’s Hospital and Department of Pediatrics, Harvard Medical School, 300 Longwood Ave, Boston, MA 02115, USA.[*2]The No.1 Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, 120 Guanghua Street, Kunming 650021, P. R. China.
推荐引用方式(GB/T 7714)：
Cao Yong-Jun,Xu Ying,Liu Bei,et al.Dioscin, a Steroidal Saponin Isolated from Dioscorea nipponica, Attenuates Collagen-Induced Arthritis by Inhibiting Th17 Cell Response.[J].The American journal of Chinese medicine.2019,47(2):423-437.doi:10.1142/S0192415X19500216.
APA：
Cao Yong-Jun,Xu Ying,Liu Bei,Zheng Xi,Wu Jian...&Wan Chun-Ping.(2019).Dioscin, a Steroidal Saponin Isolated from Dioscorea nipponica, Attenuates Collagen-Induced Arthritis by Inhibiting Th17 Cell Response..The American journal of Chinese medicine,47,(2)
MLA：
Cao Yong-Jun,et al."Dioscin, a Steroidal Saponin Isolated from Dioscorea nipponica, Attenuates Collagen-Induced Arthritis by Inhibiting Th17 Cell Response.".The American journal of Chinese medicine 47..2(2019):423-437
