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ROS-Autophagy pathway mediates monocytes-human umbilical vein endothelial cells adhesion induced by apelin-13.

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机构: [1]Institute of Pharmacy and Pharmacology, University of South China, Hengyang, China. [2]Department of Pharmacy, The Second People's Hospital of Yunnan province, Kunming, China. [3]Department of Pharmacy, The First People's Hospital of Yueyang, Hunan, China. [4]College of Pharmacy, Hunan University of Medicine, Huaihua, China.
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关键词: apelin-13 autophagy cell adhesion NOX ROS

摘要:
Apelin is the endogenous ligand of APJ receptor. Both monocytes (MCs) and human umbilical vein endothelial cells (HUVECs) express apelin and APJ, which play important roles in the physiological processes of atherosclerosis. Our previous research indicated that apelin-13 promoted MCs-HUVECs adhesion. Here, we further explore the mechanism responsible for MCs-HUVECs adhesion induced by apelin-13. Apelin-13 promoted reactive oxygen species (ROS) generation and NOX4 expression in HUVECs. Apelin-13 inducedautophagy, increased proteins beclin1 and LC3-II/I expression and induced autophagy flux in HUVECs, which was blocked by NAC, catalase and DPI. Autophagy flux induced by apelin-13 was inhibited by NAC and catalase but not hydroxychloroquine (HCQ). NAC, catalase, and DPI prevented apelin-13 induced ICAM-1 expression in HUVECs. Rapamycin enhanced MCs-HUVECs adhesion that was reversed by NAC, catalase, and DPI. Down-regulation of beclin1 and LC3 by siRNA blocked MCs-HUVECs adhesion. Apelin-13 induced atherosclerotic plaque and increased NOX4, LC3-II/I expression in ApoE-/-(HFD) mouse model. Our results demonstrated that apelin-13 induced MCs-HUVECs adhesion via a ROS-autophagy pathway. © 2018 Wiley Periodicals, Inc.

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出版当年[2018]版:
大类 | 2 区 生物
小类 | 2 区 生理学 3 区 细胞生物学
最新[2023]版:
大类 | 2 区 生物学
小类 | 2 区 生理学 3 区 细胞生物学
第一作者:
第一作者机构: [1]Institute of Pharmacy and Pharmacology, University of South China, Hengyang, China. [2]Department of Pharmacy, The Second People's Hospital of Yunnan province, Kunming, China.
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通讯机构: [1]Institute of Pharmacy and Pharmacology, University of South China, Hengyang, China. [*1]Institute of Pharmacy and Pharmacology,University of South China, Hengyang 421001,China
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