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Mesenchymal stem cells negatively regulate CD4+ T cell activation in patients with primary Sjögren syndrome through the miRNA-125b and miRNA-155 TCR pathway(Open Access)

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Pubmed体系：

收录情况： ◇ SCIE ◇ 预警期刊

作者：
BANGDONG GONG[1] * ; LING ZHENG[2] * ; ZHENHAO LU[1] ; JIASHU HUANG[1] ; JINCHENG PU[1] ; SHENGNAN PAN[1] ; MIN ZHANG[1] ; JIE LIU[3,*2] ; JIANPING TANG[1,*1] ;
机构： [1]Divisions of Rheumatology,Tongji Hospital of Tongji University School of Medicine, Shanghai 200065 [2]Divisions of Respiratory Medicine, Tongji Hospital of Tongji University School of Medicine, Shanghai 200065 [3]Center for Regenerative Medicine, The First People's Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China
出处：
DOI： 10.3892/mmr.2020.11681
ISSN：

关键词： CD4+ T cell Mesenchymal stem cells MicroRNA Primary Sjögren syndrome T cell receptor pathway

摘要：
Treatment with mesenchymal stem cells (MSCs) has been revealed to suppress CD4+ T cells and autoimmu- nity in both mouse models and patients with primary Sjögren syndrome (pSS); however, the underlying mechanism remains unclear. MicroRNAs (miRNAs or miRs) mediate CD4+ T cell activation, but the mechanism is not understood, particularly for CD4+ T cells treated with MSCs. Characterization of miRNAs may reveal pSS pathogenesis, guide MSC treatment and provide more personalized management options. The present study aimed to perform an miRNome analysis of quiescent and T cell receptor (TCR)-activated CD4+ T cells treated with MSCs via miRNA profiles and bioinformatics. Following 72 h of co-culture, MSCs inhibited TCR-induced CD4+ T cell activation and decreased IFN-γ levels. The numbers of aberrant miRNAs in pSS naïve (vs. healthy naïve), pSS activation (vs. pSS naïve), MSC treatment and pre-IFN-γ MSC treatment (vs. pSS activation) groups were 42, 55, 27 and 32, respectively. Gene enrichment analysis revealed that 259 pathways were associated with CD4+ T cell stimulation, and 240 pathways were associated with MSC treatment. Increased miRNA-7150 and miRNA-5096 and decreased miRNA-125b-5p and miRNA-22-3p levels in activated CD4+ T cells from patients with pSS were reversed by MSC treatment. Notably, the proliferation of CD4+ T cells and CD4+ IFN-γ+ cells, expression levels of miRNA-125b-5p and miRNA-155 in CD4+ T cells and supernatant IFN-γ secretion were associated with disease activity. miRNA may play a vital role in MSC treatment for activated CD4+ T cells. The results indicated that the expression levels of miRNA-125b-5p and miRNA-155 in TCR-activated CD4+ T cells from patients with pSS may provide insight regarding autoimmune diseases and offer a novel target for prospective treatment. Therefore, these results may be crucial in providing MSC treatment for pSS. © 2021 Spandidos Publications. All rights reserved.

基金：
The present study was supported by the National Natural Science Foundation of China (grant nos. 81273295, 81671598 and 81801601), the China International Medical Exchange Foundation (grant no. Z-2014-06-2-1620), the Shanghai Wu Mengchao Medical Foundation (grant no. 17YF1417200), the Clinical Research Key Program of Tongji Hospital Tongji University (grant no. ITJZD1909) and the Research Training Foundation of Tongji Hospital (grant no. GJPY1805).
语种：
外文
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WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类 | 4 区 医学
小类 | 4 区 医学：研究与实验 4 区 肿瘤学
最新[2023]版：
大类 | 3 区 医学
小类 | 4 区 医学：研究与实验 4 区 肿瘤学
JCR分区：
出版当年[2020]版：
Q3 MEDICINE, RESEARCH & EXPERIMENTAL Q4 ONCOLOGY
最新[2023]版：
Q2 ONCOLOGY Q2 MEDICINE, RESEARCH & EXPERIMENTAL

影响因子： 最新[2023版] 最新五年平均 出版当年[2020版] 出版当年五年平均 出版前一年[2019版] 出版后一年[2021版]

第一作者：
第一作者机构： [1]Divisions of Rheumatology,Tongji Hospital of Tongji University School of Medicine, Shanghai 200065
共同第一作者：
通讯作者：
通讯机构： [1]Divisions of Rheumatology,Tongji Hospital of Tongji University School of Medicine, Shanghai 200065 [3]Center for Regenerative Medicine, The First People's Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China [*1]Division of Rheumatology, Tongji Hospital of Tongji University School of Medicine, 389 Xincun Road, Putuo, Shanghai 200065, P.R. China [*2]Center for Regenerative Medicine, The First People's Hospital of Yunnan Province, 157 Jinbi Road, Kunming, Yunnan 650032, P.R. China
推荐引用方式(GB/T 7714)：
BANGDONG GONG,LING ZHENG,ZHENHAO LU,et al.Mesenchymal stem cells negatively regulate CD4+ T cell activation in patients with primary Sjögren syndrome through the miRNA-125b and miRNA-155 TCR pathway(Open Access)[J].MOLECULAR MEDICINE REPORTS.2021,23(1):doi:10.3892/mmr.2020.11681.
APA：
BANGDONG GONG,LING ZHENG,ZHENHAO LU,JIASHU HUANG,JINCHENG PU...&JIANPING TANG.(2021).Mesenchymal stem cells negatively regulate CD4+ T cell activation in patients with primary Sjögren syndrome through the miRNA-125b and miRNA-155 TCR pathway(Open Access).MOLECULAR MEDICINE REPORTS,23,(1)
MLA：
BANGDONG GONG,et al."Mesenchymal stem cells negatively regulate CD4+ T cell activation in patients with primary Sjögren syndrome through the miRNA-125b and miRNA-155 TCR pathway(Open Access)".MOLECULAR MEDICINE REPORTS 23..1(2021)

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