Background: Our study aims to analyze the expression and significance of estrogen receptor (ER) and progesterone receptor (PR) in endometrial tissues of patients with intrauterine adhesion (IUA). Methods: Fifty-four patients with IUAs examined in our hospital from January 2017 to January 2019 were selected as the research object (observation group), 54 healthy women who had physical examinations during the same period were selected as the control group, and the immunohistochemical EnVision was used. Twostep and real-time fluorescent quantitative PCR methods were used to detect the expression levels of ER and PR in the endometrial tissues of the two groups of subjects. Results: The immunohistochemical test results showed that ER's expression level in the observation group was significantly higher than that in the control group, and the difference between the two groups was statistically significant (P<0.05). The difference in PR expression levels between the two groups was insignificant (P<0.05). Real-time fluorescent quantitative PCR results showed ER and PR's expression levels in the observation group were significantly higher than those in the control group, and the difference between the two groups was statistically significant (P<0.05). Conclusions: After IUA can detect ER and PR expression, we can formulate a personalized hormone treatment plan to improve the clinical treatment effect.