机构：[1]The Fifth Department of Biological Products , Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College , Kunming , Yunnan Province , People's Republic of China.[2]The First Hospital of Yunnan Province , Kunming , Yunnan Province , People's Republic of China.
Vaccination is the practiced and accessible measure for preventing influenza infection. Because chicken embryos used for vaccine production have various insufficiencies, more efficient methods are needed. African green monkey kidney (Vero) cells are recommended by the World Health Organization (WHO) as a safe substitute for influenza vaccine production for humans. However, the influenza virus usually had low-yield in Vero cells, which limits the usage of Vero cellular vaccines. This study used 2 high-yield influenza viruses in Vero cells: A/Yunnan/1/2005Va (H3N2) and B/Yunnan/2/2005Va (B) as donor viruses. It used 3 wild strain viruses to reassort new adaptation viruses, including: A/Tianjin/15/2009(H1N1), A/Fujian/196/2009(H3N2), and B/Chongqing/1384/2010(B). These three new viruses could maintain the characteristic of high-yield in Vero cells. Furthermore, they could keep the immunogenic characteristics of the original wild influenza viruses. Importantly, these viruses were shown as safe in chicken embryo and guinea pigs assessment systems. These results provide an alternative method to produce influenza vaccine based on Vero cells.
基金：
International Cooperation Project
(2011DFR30420); Innovation team project in Yunnan Province
(2015HC027); Science and technology innovation strong province
project in Yunnan Province (2014AE008); Scientific Research Projects
of Health Care (200802023); National 863 Program (2012AA02A404);
National Science and Technology Major Project (2013ZX10004003-
003-002).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区医学
小类|3 区生物工程与应用微生物4 区免疫学
最新[2025]版：
大类|3 区医学
小类|3 区生物工程与应用微生物3 区免疫学
第一作者：
第一作者机构：[1]The Fifth Department of Biological Products , Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College , Kunming , Yunnan Province , People's Republic of China.
通讯作者：
通讯机构：[1]The Fifth Department of Biological Products , Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College , Kunming , Yunnan Province , People's Republic of China.[*1]The Fifth Department of Biological Products, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, The 935th Jiaoling Road, Kunming, Yunnan Province, People’s Republic of China, Zip Code: 650118.
推荐引用方式(GB/T 7714)：
Zhou Jian,Yang Fan,Yang Jinghui,et al.Reassortment of high-yield influenza viruses in vero cells and safety assessment as candidate vaccine strains[J].Human vaccines & immunotherapeutics.2017,13(1):111-116.doi:10.1080/21645515.2016.1231261.
APA：
Zhou Jian,Yang Fan,Yang Jinghui,Ma Lei,Cun Yina...&Liao Guoyang.(2017).Reassortment of high-yield influenza viruses in vero cells and safety assessment as candidate vaccine strains.Human vaccines & immunotherapeutics,13,(1)
MLA：
Zhou Jian,et al."Reassortment of high-yield influenza viruses in vero cells and safety assessment as candidate vaccine strains".Human vaccines & immunotherapeutics 13..1(2017):111-116
