机构：[1]Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People’s Republic of China[2]PET/CT Center, Yunan Tumor Hospital, the Third Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People’s Republic of China[3]Department of Orthopaedics, Yunan Tumor Hospital, the Third Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People’s Republic of China[4]Department of Pharmacy, the Second Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People’s Republic of China
The present study intended to demonstrate the effects of long noncoding RNA growth arrest-specific transcript 5 (GAS5) on the migration and invasion of melanoma cells. We first detected the expression of GAS5 among four kinds of melanoma cell lines, followed by constructing GAS5-knocked down and overexpressed stable cells. Next, we evaluated the effects of GAS5 on cell migration and invasion using wound healing and gelatin zymography assays. Finally, melanoma cells with different GAS5 expression were injected into nude mice, and the tumor volumes were recorded and tumor tissues were analyzed after sacrificing the mice. This study systematically examined the function of GAS5 in mediating melanoma metastasis and revealed that GAS5 plays an anticancer role in melanoma via regulating gelatinase A and B, both in vitro and in vivo.
基金：
National Science Foundation of China (number 81160246 and number 81160421) and the Science and Technology Fund of Yunnan Province (number 2013FB102).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区肿瘤学
最新[2023]版：
大类|4 区医学
小类|3 区生物工程与应用微生物4 区肿瘤学
第一作者：
第一作者机构：[1]Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People’s Republic of China[2]PET/CT Center, Yunan Tumor Hospital, the Third Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People’s Republic of China[*1]Department of Biochemistry and Molecular Biology, Kunming Medical University, 1168 Yuhua Street, Chenggong District, Kunming, Yunnan, 150080, People’s Republic of China
推荐引用方式(GB/T 7714)：
Long Chen,Huixin Yang,Yanbin Xiao,et al.LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo[J].OncoTargets and therapy.2016,9:4075-87.doi:10.2147/OTT.S98203.
APA：
Long Chen,Huixin Yang,Yanbin Xiao,Xiaoxia Tang,Yuqian Li...&Yuechun Zhu.(2016).LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo.OncoTargets and therapy,9,
MLA：
Long Chen,et al."LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo".OncoTargets and therapy 9.(2016):4075-87
