机构：[1]Department of Biochemistry and Molecular Biology of Kunming Medical University, Yunnan, P.R. China.[2]Department of Orthopaedic Surgery, The Third Affiliated Hospital of Kunming Medical University, Yunnan, P.R. China.[3]Department of Pharmacy, The Second Affiliated Hospital of Kunming Medical University, Yunnan, P.R. China.
The present study evaluated the effects of long non-coding RNA GAS5 on the migration and invasion of melanoma cells. Using the SK-Mel‑110 melanoma cell line, we stably expressed GAS5, visualized the distribution of GAS5 by RNA fluorescence in situ hybridization (FISH) and examined changes in cell migration and invasion with Transwell assays. In GAS5 overexpressed SK-Mel‑110 cells, migrated and invaded cells decreased by 65.3 and 55.6%, respectively. Moreover, the MMP2 protein level, and its activity was downregulated by 67.9 and 15.8%, respectively. Overexpressing lncRNA GAS5 inhibited the migration and invasion ability of melanoma SK-Mel‑110 cells, partially by decreasing the MMP2 expression and its activity. This study is the first to reveal a potential relationship between lncRNA GAS5 and the migration and invasion of melanoma.
基金：
National Science Foundation
of China (nos. 81160246 and 81160421) and the Science and
Technology Fund of Yunnan Province (no. 2013FB102).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|3 区医学
小类|3 区肿瘤学
最新[2023]版：
大类|3 区医学
小类|3 区肿瘤学
第一作者：
第一作者机构：[1]Department of Biochemistry and Molecular Biology of Kunming Medical University, Yunnan, P.R. China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Biochemistry and Molecular Biology of Kunming Medical University, Yunnan, P.R. China.[*1]Department of Biochemistry and Molecular Biology of Kunming Medical University, Yunnan, P.R. China
推荐引用方式(GB/T 7714)：
Chen Long,Yang Huixin,Xiao Yanbin,et al.Lentiviral-mediated overexpression of long non-coding RNA GAS5 reduces invasion by mediating MMP2 expression and activity in human melanoma cells[J].International journal of oncology.2016,48(4):1509-18.doi:10.3892/ijo.2016.3377.
APA：
Chen Long,Yang Huixin,Xiao Yanbin,Tang Xiaoxia,Li Yuqian...&Zhu Yuechun.(2016).Lentiviral-mediated overexpression of long non-coding RNA GAS5 reduces invasion by mediating MMP2 expression and activity in human melanoma cells.International journal of oncology,48,(4)
MLA：
Chen Long,et al."Lentiviral-mediated overexpression of long non-coding RNA GAS5 reduces invasion by mediating MMP2 expression and activity in human melanoma cells".International journal of oncology 48..4(2016):1509-18
