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20(R)-ginsenoside Rg3 protects against focal cerebral ischemia‒reperfusion injury by suppressing autophagy via PI3K/Akt/mTOR signaling pathway

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资源类型：
Pubmed体系：
作者：
Tao Daiju[1] * ; Li Fajing[1,2] * ; Zhang Xiaochao[1] * ; Guo Hui[1,3] ; Yang Renhua[1] ; Yang Yuan[1] ; Zhang Li[1] ; Shen Zhiqiang[1] ; Teng Jia[1,3] ; Chen Peng[1] ; He Bo[1] ;
机构： [1]School of Pharmaceutical Science, Yunnan Key Laboratory of Pharmacology for Natural Products, NHC Key Laboratory of Drug Addiction Medicine, Kunming Medical University, Kunming, 650500, PR China. [2]The First People's Hospital of Liangshan Yi Autonomous Prefecture, XiChang, Sichuan Province, 615000, PR China. [3]Department of Pharmacology, Haiyuan College, Kunming Medical University, 650106, PR China.
出处：
DOI： 10.1016/j.neuropharm.2024.110226
ISSN：

关键词： 20(R)-Ginsenoside Rg3 Cerebral ischemia‒reperfusion Autophagy PI3K/Akt signaling pathway

摘要：
This study aimed to investigate the effect of 20(R)-ginsenoside Rg3 on autophagy induced by cerebral ischemia‒reperfusion injury (CIRI) in rats and explore its regulation of the PI3K/Akt signaling pathway.Middle cerebral artery occlusion/reperfusion (MCAO/R) in male rats was injected intraperitoneally with 20(R)-ginsenoside Rg3 (5, 10, 20 mg/kg) 12 h before modeling, 2 h after ischemia and 12 h after reperfusion. Neurobehavioral and neuronal morphological changes were detected 24 h after brain I/R. In vitro, the OGD/R-induced injury model is replicated in PC12 cells and different concentrations of 20(R)-ginsenoside Rg3 are administered to observe its effects on cell viability and autophagy and PI3K/Akt/mTOR-related protein expression.Our findings suggest that treatment with 20 mg/kg 20(R)-ginsenoside Rg3 significantly attenuated the neuronal injury, as evidenced by a decreased number of damaged neurons, reduced dissolution of Nissl corpuscles, a fewer autophagosomes, and downregulated expression of Beclin1 and LC3-II/I compared with the MCAO/R group. Furthermore, 20(R)-ginsenoside Rg3 treatment significantly upregulated the expression of p62, p-PI3K, p-AKT, and p-mTOR. In vitro, 20(R)-ginsenoside Rg3 significantly improved the survival rate of cells following OGD/R and markedly attenuated the LY294002 and OGD/R-induced upregulation of Beclin1 and LC3 gene expression. Moreover, 20(R)-ginsenoside Rg3 could rescued the LY294002 and OGD/R-induced downregulation of p62, p-PI3K, p-AKT, and p-mTOR expression.20(R)-ginsenoside Rg3 attenuates neuronal injury and motor dysfunction following ischemia-reperfusion by inhibiting the activation of autophagy, and its mechanism is related to the upregulation of the PI3K/Akt/mTOR signaling pathway.Copyright © 2024 Elsevier Ltd. All rights reserved.

基金：
This work was supported by the National Natural Science Foundation of China (No. 82260271, 81860665), the Joint Fund for the Department of Science and Technology of Yunnan Province-Kunming Medical University (No. 202001AY070001-157), and First-Class Discipline Team of Kunming Medical University (No. 2024XKTDTS12), NHC Key Lab of Drug Addiction Medicine（Kunming Medical University）Open Projects Fund（Project NO. KN202422).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2025]版：
无
最新[2025]版：
大类 | 2 区 医学
小类 | 2 区 药学 3 区 神经科学
第一作者：
第一作者机构： [1]School of Pharmaceutical Science, Yunnan Key Laboratory of Pharmacology for Natural Products, NHC Key Laboratory of Drug Addiction Medicine, Kunming Medical University, Kunming, 650500, PR China.
共同第一作者：
通讯作者：
通讯机构： [1]School of Pharmaceutical Science, Yunnan Key Laboratory of Pharmacology for Natural Products, NHC Key Laboratory of Drug Addiction Medicine, Kunming Medical University, Kunming, 650500, PR China. [3]Department of Pharmacology, Haiyuan College, Kunming Medical University, 650106, PR China.
推荐引用方式(GB/T 7714)：
Tao Daiju,Li Fajing,Zhang Xiaochao,et al.20(R)-ginsenoside Rg3 protects against focal cerebral ischemia‒reperfusion injury by suppressing autophagy via PI3K/Akt/mTOR signaling pathway[J].Neuropharmacology.2025,263:110226.doi:10.1016/j.neuropharm.2024.110226.
APA：
Tao Daiju,Li Fajing,Zhang Xiaochao,Guo Hui,Yang Renhua...&He Bo.(2025).20(R)-ginsenoside Rg3 protects against focal cerebral ischemia‒reperfusion injury by suppressing autophagy via PI3K/Akt/mTOR signaling pathway.Neuropharmacology,263,
MLA：
Tao Daiju,et al."20(R)-ginsenoside Rg3 protects against focal cerebral ischemia‒reperfusion injury by suppressing autophagy via PI3K/Akt/mTOR signaling pathway".Neuropharmacology 263.(2025):110226

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