机构：[1]Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China中山大学附属第一医院[2]Department of Pediatrics, Guangdong General Hospital, Guangdong Neuroscience Institute, Guangdong Academy of Medical Sciences,广东省人民医院[3]Department of Neurology, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China昆明医科大学附属第一医院[4]Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China[5]Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou 510085, Guangdong Province, China
In this study, recombinant baculovirus carrying the microdystrophin and beta-catenin genes was used to infect adipose-derived stem cells from a dystrophin-utrophin double knock-out mouse. Results showed that, after baculovirus transgene infection, microdystrophin and beta-catenin genes were effectively expressed in adipose-derived stem cells from the dystrophin-utrophin double khock-out mouse. Furthermore, this transgenic expression promoted adipose-derived stem cell differentiation into muscle cells, but inhibited adipogenic differentiation. In addition, protein expression related to the microdystrophin and Wnt/beta-catenin signaling pathway was upregulated. Our experimental findings indicate that baculovirus can successfully deliver the microdystrophin and beta-catenin genes into adipose-derived stem cells, and the microdystrophin and Wnt/beta-catenin signaling pathway plays an important role in myogenesis of adipose-derived stem cells in the dystrophin-utrophin double knock-out mouse.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [30370510, 30170337, 30400322, 30870851]; CMB Fund [4209347]; State Ministry of Public Health [2001321]; Fok Ying Tung Education FoundationFok Ying Tung Education Foundation [91029]; Key Projects in the National Science and Technology Pillar Program [2006BAI05A07]
第一作者机构：[1]Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China[2]Department of Pediatrics, Guangdong General Hospital, Guangdong Neuroscience Institute, Guangdong Academy of Medical Sciences,
通讯作者：
通讯机构：[1]Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China[*1]Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
推荐引用方式(GB/T 7714)：
Li Qiuling,Zhai Qiongxiang,Geng Jia,et al.Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse[J].NEURAL REGENERATION RESEARCH.2012,7(22):1695-1702.doi:10.3969/j.issn.1673-5374.2012.22.003.
APA：
Li, Qiuling,Zhai, Qiongxiang,Geng, Jia,Zheng, Hui,Chen, Fei...&Zhang, Cheng.(2012).Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse.NEURAL REGENERATION RESEARCH,7,(22)
MLA：
Li, Qiuling,et al."Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse".NEURAL REGENERATION RESEARCH 7..22(2012):1695-1702
