Inclusion complexes between chrysin and amino-appended beta-cyclodextrins (ACDs): Binding behavior, water solubility, in vitro antioxidant activity and cytotoxicity
机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, 650500 Kunming, China[2]Jiangsu Xinchen Pharmaceutical Co., LTD, 222047 Lianyungang, China[3]School of Pharmacy, Jiangsu Key Laboratory for Bioresources of Saline Soils, Yancheng Teachers University, 224007 Yancheng, China[4]Faculty of Medicine, Kunming University of Science and Technology, Kunming 650500, China[5]Research Centre for Pharmaceutical Care and Quality Management, First People's Hospital of Yunnan Province, Kunming 650500, China云南省第一人民医院[6]Current address: Faculty of Science and Engineering, University of Groningen, Groningen, the Netherlands.
Solid inclusion complexes between chrysin and four amino-appended beta-cyclodextrins (ACDs) were prepared by suspension method and characterized in solid and solution states by kinds of analytical methods. The scanning electron microscopy (SEM) showed distinct micro-morphologies of them. Thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analysis revealed their unique thermal properties, such as decomposition temperatures and endothermic points. Powder X-ray diffractometry (XRD) analysis disclosed their unique crystal patterns. Their nuclear magnetic resonance (NMR) analyses provided the variations of chemical shifts before and after the formation of inclusion complexes. Their binding stability constants (Ks) were 574, 842, 704, and 474 L.mol(-1), respectively, as determined by spectral titration. A 1:1 inclusion mode with self-assembly of their amino side chains inside the ACD cavity was proposed based on Job plot and 2D-ROESY experiments. Water solubility of chrysin was promoted up to 4411.98 mu g.mL(-1) after formation of inclusion complexes with ACDs, better than that of beta-CD and its derivatives, ie., HP- and SBE-beta-CD. In vitro antioxidant activity of chrysin was also improved after inclusion complexation by the DPPH scavenging assay. Furthermore, in vitro cytotoxicity of solid inclusion complexes towards three human cancer cell lines, A549, HT-29 and HCT116 were enhanced significantly.
基金：
This work was financial supported by the National Natural Science
Foundation of China (NNSFC) (Nos. 21642001 & 81560601), Yunnan
Applied Basic Research Projects (Nos. 2018FB018 & 2018FA047) and
Natural Science Foundation of Jiangsu Province (BK20160443), which
are gratefully acknowledged.
第一作者机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, 650500 Kunming, China
共同第一作者：
通讯作者：
通讯机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, 650500 Kunming, China[*1]Faculty of Life Science and Technology, Kunming University of Science and Technology, 650500 Kunming, China.
推荐引用方式(GB/T 7714)：
Song Shuang,Gao Kai,Niu Raomei,et al.Inclusion complexes between chrysin and amino-appended beta-cyclodextrins (ACDs): Binding behavior, water solubility, in vitro antioxidant activity and cytotoxicity[J].MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS.2020,106:doi:10.1016/j.msec.2019.110161.
APA：
Song, Shuang,Gao, Kai,Niu, Raomei,Wang, Jin,Zhang, Jihong...&Liao, Xiali.(2020).Inclusion complexes between chrysin and amino-appended beta-cyclodextrins (ACDs): Binding behavior, water solubility, in vitro antioxidant activity and cytotoxicity.MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,106,
MLA：
Song, Shuang,et al."Inclusion complexes between chrysin and amino-appended beta-cyclodextrins (ACDs): Binding behavior, water solubility, in vitro antioxidant activity and cytotoxicity".MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 106.(2020)
