Physiological and Metabolomics Analyses Reveal the Roles of Fulvic Acid in Enhancing the Production of Astaxanthin and Lipids in Haematococcus pluvialis under Abiotic Stress Conditions
机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China[2]The First People’s Hospital of Yunnan, Kunming 650100, China[3]Yunnan Academy of Forestry, Kunming 650051, China
In this study, it was found that fulvic acid (FA) enhanced the contents of astaxanthin and lipids in Haematococcus pluvialis under high light and nitrogen starvation conditions by 2- and 1.2-fold, respectively. Meanwhile, the carbohydrate and chlorophyll contents were decreased by FA induction, whereas the levels of reactive oxygen species (ROS) and glutathione (GSH) as well as the expression of astaxanthin and lipid biosynthetic genes were increased. To further explore the interrelation between FA and the biosynthesis of astaxanthin and lipids, a metabolomics analysis of H. pluvialis by combined FA and abiotic stress exposure was conducted by using LC-MS/MS. The contents of some cytoprotective metabolites and signal molecules, including n-maltose, succinate, malic acid, melatonin (MT), and some amino acids, were increased under FA induction and abiotic stress conditions. These metabolites are intermediates in the TCA cycle and Calvin cycle, providing more precursors for the synthesis of astaxanthin and lipids. Moreover, the signal molecules might contribute to enhancing the abiotic stress tolerance. This study provided new insights into the regulatory mechanism of FA on astaxanthin and lipid accumulation in H. pluvialis.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21766012]; Key Science and Technology Project of Yunnan Province, China [2018ZG003]
第一作者机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhao Yongteng,Xing Hailiang,Li Xingyu,et al.Physiological and Metabolomics Analyses Reveal the Roles of Fulvic Acid in Enhancing the Production of Astaxanthin and Lipids in Haematococcus pluvialis under Abiotic Stress Conditions[J].JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.2019,67(45):12599-12609.doi:10.1021/acs.jafc.9b04964.
APA：
Zhao, Yongteng,Xing, Hailiang,Li, Xingyu,Geng, Shuxiang,Ning, Delu...&Yu, Xuya.(2019).Physiological and Metabolomics Analyses Reveal the Roles of Fulvic Acid in Enhancing the Production of Astaxanthin and Lipids in Haematococcus pluvialis under Abiotic Stress Conditions.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67,(45)
MLA：
Zhao, Yongteng,et al."Physiological and Metabolomics Analyses Reveal the Roles of Fulvic Acid in Enhancing the Production of Astaxanthin and Lipids in Haematococcus pluvialis under Abiotic Stress Conditions".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67..45(2019):12599-12609
农林科学