机构：[1]Department of Clinical Laboratory Medicine, the First People’s Hospital of Yunnan Province, No. 157, Jinbi Road, Kunming, Yunnan Province, China医技片检验科云南省第一人民医院[2]Department of Reproductive Gynecology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan Province, China云南省第一人民医院生殖妇科外科片血管外科[3]Department of Clinical Laboratory Medicine, The First People’s Hospital of Yunnan Province, Kunming, Yunnan Province, China医技片检验科云南省第一人民医院
Background: The molecular mechanisms underlying cervical cancer require elucidation to identify novel therapeutic targets. Apurinic/apyrimidinic endodeoxyribonuclease 1 (APE1) is a multifunctional apurinic/apyrimidinic (AP) endonuclease that influences the transcription of many cancer-related genes via microRNome regulation. Herein, we examine the role of miR-92b-3p (hereinafter miR-92b), whose processing may be regulated by APE1, in cervical cancer progression. Methods: APE1's processing of miR-92b from its pri-miR form was measured by a quantitative reverse transcription polymerase chain reaction (qRT-PCR)-based ratio. APE1's endonuclease activity was measured with AP-site incision assays. APE1-DROSHA interaction was studied with immunofluorescence, confocal and proximity ligation analyses. The miR-92b's targeting of low-density lipoprotein receptor (LDLR) was investigated with luciferase reporter assays. The miR-92b mimics and shRNA-based miR-92b silencing, as well as LDLR overexpression and short interfering RNA (siRNA)-based LDLR silencing, were employed in CaSki and SiHa cervical cancer cells. Cell proliferation and chemosensitivity to paclitaxel and cisplatin were assayed. Cell-cycle progression and apoptosis were assessed by flow cytometry. Tumor growth was studied in a murine xenograft model. Results: APE1's endonuclease activity, via association with the DROSHA-processing complex, is necessary for processing mature miR-92b, thereby regulating expression of miR-92b's direct target LDLR. The miR-92b promotes cell proliferation in vitro and in vivo, promotes cell-cycle progression, and reduces apoptosis and chemosensitivity. LDLR silencing recapitulated miR-92b's transformative effects, while LDLR overexpression rescued these effects. Conclusions: APE1 enhances miR-92b processing, thereby suppressing LDLR expression and enhancing cervical carcinoma progression. Our identification of the novel APE1-miR-92b-LDLR axis improves our understanding of the complex pathogenesis of cervical carcinoma and reveals a novel therapeutic strategy for combating this disease.
基金：
Yunnan Provincial Health and Family Planning Commission Medical Discipline Leaders Training Program [D-201670, 2018IC106]
第一作者机构：[1]Department of Clinical Laboratory Medicine, the First People’s Hospital of Yunnan Province, No. 157, Jinbi Road, Kunming, Yunnan Province, China
通讯作者：
推荐引用方式(GB/T 7714)：
Sun Yi,Feng Yun,Zhang Guiqian,et al.The endonuclease APE1 processes miR-92b formation, thereby regulating expression of the tumor suppressor LDLR in cervical cancer cells[J].THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY.2019,11:doi:10.1177/1758835919855859.
APA：
Sun, Yi,Feng, Yun,Zhang, Guiqian&Xu, Ya.(2019).The endonuclease APE1 processes miR-92b formation, thereby regulating expression of the tumor suppressor LDLR in cervical cancer cells.THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY,11,
MLA：
Sun, Yi,et al."The endonuclease APE1 processes miR-92b formation, thereby regulating expression of the tumor suppressor LDLR in cervical cancer cells".THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY 11.(2019)
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