机构：[1]Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China[2]Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China[3]The First People's Hospital of Yunnan Province, 157 Jinbi Road, Kunming 650032, China云南省第一人民医院[4]Institute of Biomedical Sciences, Minhang hospital, Fudan University, 170 Xinsong Road, Shanghai 201199, China[5]State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, 2005 Songhu Road, Shanghai 200433, China
A-Raf is a member of the Raf kinase family. Unlike B-Raf and C-Raf, the functions of A-Raf remain obscure. To gain more insight into the biological functions of A-Raf, we investigated the A-Raf interactome using proteomics. We found 132 proteins that interact with A-Raf and confirmed the interaction of 12 of these proteins with A-Raf by western blotting. Our data suggested that A-Raf regulates apoptosis, RNA catabolism, GTPase activity, and cell adhesion by interacting with proteins located in different cellular compartments. We identified all ten hallmarks of cancer in these interacting proteins, suggesting that A-Raf is involved in carcinogenesis. Our results also indicated that A-Raf may play a role in different diseases and signaling pathways. These findings have identified potential regulators of A-Raf and provide a systemic insight into its biological functions.
基金：
NSFCNational Natural Science Foundation of China [81460253, 81460417, 81560455, 81760264, 81473342, 31660099, U1302225]; Yunnan Applied Basic Research Projects-Union foundation Management System [2017FE468-241]; Scientific Research Grant of Education Department of Yunnan Province, China [2015Y046]; University Based Provincial Key Laboratory of Screening and Utilization of Targeted Drugs
第一作者机构：[1]Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China[2]Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Xin-Yu,Guo Hui,Han Bing,et al.Revealing A-Raf functions through its interactome[J].BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS.2018,1866(8):849-856.doi:10.1016/j.bbapap.2018.05.009.
APA：
Zhang, Xin-Yu,Guo, Hui,Han, Bing,Zhang, Xu-Min,Huang, Ying...&Xu, Tian-Rui.(2018).Revealing A-Raf functions through its interactome.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1866,(8)
MLA：
Zhang, Xin-Yu,et al."Revealing A-Raf functions through its interactome".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1866..8(2018):849-856
