机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500[2]Department of Endocrinology, The Affiliated Hospital of Kunming University of Science and Technology,The First People's Hospital of Yunnan, Kunming, Yunnan 650032内科片内分泌科云南省第一人民医院[3]Department of Cadre Ward,WISCO General Hospital, Wuhan, Hubei 430080[4]Institute of Basic and Clinical Medicine,Center of Clinical Molecular Biology of Yunnan, The Affiliated Hospital of Kunming University of Science and Technology,The First People's Hospital of Yunnan, Kunming, Yunnan 650032, P.R. China云南省第一人民医院
The present study aimed to investigate the role of the extracellular signal-regulated kinase (ERK) 1/2 signal transduction pathway in glucose-stimulated insulin secretion in beta-TC6 mouse pancreatic cells. Insulin production by beta-TC6 cells was stimulated with various concentrations of glucose, which was dose-dependently inhibited by mitogen-activated protein kinase inhibitor PD98059, as indicated by a radioimmunoassay. Furthermore, glucose stimulation enhanced the phosphorylation of ERK1/2, which was dose-dependently inhibited by PD98059, as indicated by western blot analysis. These results indicated that the activation of the ERK1/2 signal transduction pathway may have an important role in glucose-stimulated insulin secretion in beta-TC6 cells.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81560135]
第一作者机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500[2]Department of Endocrinology, The Affiliated Hospital of Kunming University of Science and Technology,The First People's Hospital of Yunnan, Kunming, Yunnan 650032
通讯作者：
通讯机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500[2]Department of Endocrinology, The Affiliated Hospital of Kunming University of Science and Technology,The First People's Hospital of Yunnan, Kunming, Yunnan 650032[*1]Department of Endocrinology, The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan, 157 Jinbi Road, Kunming, Yunnan 650032, P.R. China[*2]Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming South Road, Cheng Gong District, Kunming, Yunnan 650500, P.R. China
推荐引用方式(GB/T 7714)：
Niu Ben,Liu Lijuan,Su Heng,et al.Role of extracellular signal-regulated kinase 1/2 signal transduction pathway in insulin secretion by beta-TC6 cells[J].MOLECULAR MEDICINE REPORTS.2016,13(5):4451-4454.doi:10.3892/mmr.2016.5053.
APA：
Niu, Ben,Liu, Lijuan,Su, Heng,Xia, Xueshan,He, Qiu...&Yan, Xinming.(2016).Role of extracellular signal-regulated kinase 1/2 signal transduction pathway in insulin secretion by beta-TC6 cells.MOLECULAR MEDICINE REPORTS,13,(5)
MLA：
Niu, Ben,et al."Role of extracellular signal-regulated kinase 1/2 signal transduction pathway in insulin secretion by beta-TC6 cells".MOLECULAR MEDICINE REPORTS 13..5(2016):4451-4454
