机构：[1]Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, Yunnan, China[2]National-Local Joint Engineering Research Center of Entomoceutics, Dali University, Dali, Yunnan, China[3]Yunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics, Dali University, Dali, Yunnan, China[4]Good Doctor Pharmaceutical Group, Chengdu, Sichuan, China[5]Department of Gastroenterology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, China内科片消化内科云南省第一人民医院
Ulcerative colitis (UC) is a chronic inflammatory disease of intestinal tract, andPeriplaneta americanahas been found to be effective in the treatment for UC. The purpose of the study was to investigate the therapeutic effect ofPeriplaneta americanaextract Ento-A on UC in rats induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) and to explore its mechanism. The Sprague-Dawley (SD) rats were randomly divided into normal control group; TNBS-treated group; sulfasalazine (SASP) treated group; Ento-A low- (50 mg/kg), medium- (100 mg/kg), and high-dose (200 mg/kg) groups, respectively. The UC model of rats was induced via TNBS. Disease activity index (DAI) was used to evaluate the severity of UC in rats. The macroscopic and microscopic damages of colon were accessed by colon mucosa damage index (CMDI) and histopathological score (HS), respectively. The levels of interleukin-4 (IL-4), interleukin-17 (IL-17), tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma (IFN-gamma) in serum and the contents of myeloperoxidase (MPO), transforming growth factor-beta 1 (TGF-beta 1), and epidermal growth factor (EGF) in colonic mucosa were measured by enzyme-linked immunosorbent assay (ELISA). Compared with the normal control group, the TNBS-treated group showed increase in DAI, CMDI, HS, IL-17, TNF-alpha, IFN-gamma as well as MPO and decrease in the levels of IL-4, EGF, and TGF-beta 1. However, Ento-A-administrated groups reversed the changes in the DAI, CMDI, HS, and the cytokines caused by TNBS. The study indicates thatPeriplaneta americanaextract Ento-A can effectively alleviate the inflammation in TNBS-induced UC of rats, and the mechanism of that may be related to restoring the balance of T helper 1 (Th1)/Th2/Th17/T regulatory (Treg) cytokines.
基金：
This study is supported by several funds as the
following: The National Natural Science Fundation of
China (No. 81660605, 81860742, 81860765, 81660186);
Yunnan Fundamental Research Projects (No. 2017FH001-
108, 2018FB143, 2019FH001-003 and 2020FB022);
Innovation Team of Dali University for Advanced
Pharmaceutics of Entomological Bio-pharmacy R&D (No.
ZKLX2019101); Innovative Team of Dali University for
Digital Development of Medicinal Insects and Arachnids
Resources (No. ZKLX2020303); and Special Fund for
Traditional Chinese Medicine Pieces of Yunnan Industry
and Information Commission (No. 2019-YG-067);
Yunnan Provincial Department of Science and Technology - Kunming Medical University Joint Special Project on
Applied Basic Research (No. 2017FE468 (-009)).
第一作者机构：[1]Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, Yunnan, China[2]National-Local Joint Engineering Research Center of Entomoceutics, Dali University, Dali, Yunnan, China
共同第一作者：
通讯作者：
通讯机构：[1]Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, Yunnan, China[2]National-Local Joint Engineering Research Center of Entomoceutics, Dali University, Dali, Yunnan, China[3]Yunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics, Dali University, Dali, Yunnan, China[*1]Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, No.22, Wanhua Road, Xiaguan Town, Dali 671000, Yunnan, China[*2]Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, No.22, Wanhua Road, Xiaguan Town, Dali 671000, Yunnan, China.
推荐引用方式(GB/T 7714)：
Che Yi-Hao,Yang Zhi-Bin,Zhang Han-Chao,et al.Study on the effect of Periplaneta americanaon ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid[J].EUROPEAN JOURNAL OF INFLAMMATION.2020,18:doi:10.1177/2058739220942629.
APA：
Che, Yi-Hao,Yang, Zhi-Bin,Zhang, Han-Chao,Wu, Xiu-Mei,Sun, Min-Zhe...&Zhang, Cheng-Gui.(2020).Study on the effect of Periplaneta americanaon ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid.EUROPEAN JOURNAL OF INFLAMMATION,18,
MLA：
Che, Yi-Hao,et al."Study on the effect of Periplaneta americanaon ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid".EUROPEAN JOURNAL OF INFLAMMATION 18.(2020)
