机构：[1]The First Affiliated Hospital, Genetic Testing Center, Yunnan, China[2]Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Yunnan, China.[3]Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, and National-Local Joint Engineering Research Center of Entomoceutics, Yunnan, China.[4]Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Yunnan, China[5]National-Local Joint Engineering Research Center of Entomoceutics, Yunnan, China.[6]The First Affiliated Hospital, Genetic Testing Center, and Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Yunnan, China.
To investigate the effects of Periplaneta americana L. on ulcerative colitis (UC) induced by a combination of chronic stress (CS) and 2,4,6-trinitrobenzene sulfonic acid enema (TNBS) in rats.The experiment UC model with CS was established in rats by a combination of chronic restraint stress, excess failure, improper, and TNBS. The body weight, disease activity index (DAI), colonic mucosal injury index (CMDI), histopathological score (HS) and pro-inflammatory mediators were measured. The content of corticotropin-releasing hormone (CRH) in hypothalamus or adrenocorticotropic hormone (ACTH) and corticosteroids (CORT) in plasma were evaluated by enzyme-linked immunosorbent assay. The proportion of T lymphocyte subsets was detected by flow cytometry, and gut microbiota was detected by 16S rDNA amplicon sequencing.Weight loss, DAI, CMDI, HS and proinflammatory mediators were reversed in rats by P. americana L. treatment after UC with CS. Increased epidermal growth factor (EGF) was observed in P. americana L. groups. In addition, P. americana L. could reduce the content of CRH and ACTH and regulate the ratio of CD3+, CD3+CD8+ and CD3+CD4+CD25+/CD4+ in spleen. Comparably, P. americana L. changes composition of gut microbiota.The ethanol extract of Periplaneta Americana L. improves UC induced by a combination of CS and TNBS in rats.
基金：
The National Natural Science Foundation of China
[https://doi.org/10.13039/501100001809]
Grants no. 81660605, 81860742, 81860765
Yunnan Fundamental Research Projects
Grants no. 2018FB143, 2019FH001-003, 2019FH001-004
The Special Program of Science and Technology of Yunnan Province
Grant no.202002AA100007
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|4 区医学
小类|4 区外科
最新[2023]版：
大类|4 区医学
小类|4 区外科
第一作者：
第一作者机构：[1]The First Affiliated Hospital, Genetic Testing Center, Yunnan, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Jing-Na,Sun Min-Zhe,Liu Heng,et al.The ethanol extract of Periplaneta Americana L. improves ulcerative colitis induced by a combination of chronic stress and TNBS in rats[J].Acta cirurgica brasileira.2022,37(5):e370505.doi:10.1590/acb370505.
APA：
Zhang Jing-Na,Sun Min-Zhe,Liu Heng,Zhang Han-Chao,Xiao Huai...&Zhao Hai-Rong.(2022).The ethanol extract of Periplaneta Americana L. improves ulcerative colitis induced by a combination of chronic stress and TNBS in rats.Acta cirurgica brasileira,37,(5)
MLA：
Zhang Jing-Na,et al."The ethanol extract of Periplaneta Americana L. improves ulcerative colitis induced by a combination of chronic stress and TNBS in rats".Acta cirurgica brasileira 37..5(2022):e370505
