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Circ-AK2 is associated with preeclampsia and regulates biological behaviors of trophoblast cells through miR-454-3p/THBS2.

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机构： [1]Reproductive Medicine Center, Jingmen No.1 People’s Hospital, Jingmen, Hubei, China [2]Maternity Department, Jingmen No.1 People’s Hospital, Jingmen, Hubei, China [3]School of Clinical Medicine, Kunming Medical University, Kunming, Yunnan, China [4]Postgraduate Training Basement of Jinzhou Medical University, Taihe Hospital Hubei University of Medicine, Shiyan, Hubei, China

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关键词： Circ-AK2 miR-454–3p THBS2 Preeclampsia Biological behaviors

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Circ-AK2 has been found to be differentially expressed in PE placenta tissues, however, the role and the underlying molecular mechanisms of circ-AK2 in PE remain poorly known. The expression of circ-AK2, miR-454-3p, and THBS2 mRNA was detected using quantitative real-time polymerase chain reaction. Protein levels of CyclinD1, MMP-9 and THBS2 were measured using Western blot. Cell proliferation, migration, and invasion were analyzed by 3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-di-phenytetrazoliumromide (MTT) assay and transwell assay. The interaction between miR-454-3p and circ-AK2 or THBS2 was analyzed by the dual-luciferase reporter assay. Circ-AK2 was highly expressed in placental tissues of PE, and overexpression of circ-AK2 inhibited trophoblast cell proliferation, migration and invasion. Circ-AK2 directly bound to miR-454-3p, and miR-454-3p overexpression reversed the inhibitory action of circ-AK2 in biological functions of trophoblast cells. MiR-454-3p was lowly expressed in placental tissues of PE, and directly regulated THBS2 expression in a targeted manner. Silencing miR-454-3p suppressed the proliferating, migratory, and invasive abilities of trophoblast cells, while this condition was abolished by THBS2 knockdown. Besides, we also proved circ-AK2 could regulate THBS2 expression via miR-454-3p. Circ-AK2 inhibited the proliferation, migration and invasion of trophoblast cells via targeting miR-454-3p/THBS2 axis, suggesting a novel insight into the etiology of PE and a potential therapeutic target for PE treatment. Copyright © 2020 Elsevier Ltd. All rights reserved.

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出版当年[2020]版：

大类 | 3 区医学

小类 | 2 区发育生物学 3 区妇产科学 3 区生殖生物学

最新[2023]版：

大类 | 2 区医学

小类 | 2 区发育生物学 2 区妇产科学 2 区生殖生物学

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第一作者机构： [1]Reproductive Medicine Center, Jingmen No.1 People’s Hospital, Jingmen, Hubei, China

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通讯机构： [*1]Maternity Department, Jingmen No.1 People’s Hospital, No. 167 Xiangshan Avenue, Jingmen, 448000, Hubei, China

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