机构：[1]Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058[2]State Key Laboratory and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou 310003浙江大学医学院附属第一医院[3]Research Center of Molecular Medicine of Yunnan Province, Kunming University of Science and Technology, Kunming 650500, China
Negri bodies (NBs) are formed in the cytoplasm of rabies virus (RABV)-infected cells and are accompanied by a number of host factors to NBs, in which replication and transcription occur. Here, it was found that chaperonin containing TCP-1 subunit alpha (CCT) relocalizes to NBs in RABV-infected cells, and that cotransfection of nucleo- and phospho-proteins of RABV is sufficient to recruit CCT to the NBs' structure. Inhibition of CCT expression by specific short hairpin RNA knockdown inhibited the replication and transcription of RABV. Therefore, this study showed that the host factor CCT is associated with RABV infection and is very likely required for efficient virus transcription and replication.
基金：
National Special Fund for Public Welfare Industry [201103032]; National Key Technologies Research and Development Program of ChinaNational Key Technology R&D Program [2010BAD04B01]; Applied Basic Research Projects of Yunnan Province [KKS0201326002]; National High-Tech program of ChinaNational High Technology Research and Development Program of China [2012AA101303]
第一作者机构：[1]Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058[2]State Key Laboratory and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou 310003[3]Research Center of Molecular Medicine of Yunnan Province, Kunming University of Science and Technology, Kunming 650500, China
共同第一作者：
通讯作者：
通讯机构：[1]Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058[2]State Key Laboratory and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou 310003[*1]Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China
推荐引用方式(GB/T 7714)：
Zhang Jinyang,Ye Chengjin,Ruan Xizhen,et al.The chaperonin CCT alpha is required for efficient transcription and replication of rabies virus[J].MICROBIOLOGY AND IMMUNOLOGY.2014,58(10):590-599.doi:10.1111/1348-0421.12186.
APA：
Zhang, Jinyang,Ye, Chengjin,Ruan, Xizhen,Zan, Jie,Xu, Yunbin...&Zhou, Jiyong.(2014).The chaperonin CCT alpha is required for efficient transcription and replication of rabies virus.MICROBIOLOGY AND IMMUNOLOGY,58,(10)
MLA：
Zhang, Jinyang,et al."The chaperonin CCT alpha is required for efficient transcription and replication of rabies virus".MICROBIOLOGY AND IMMUNOLOGY 58..10(2014):590-599
