详情页 - 云南省第一人民医院机构库

当前位置：首页 > 详情页

Multicentre laboratory validation of the nitrate reductase assay using liquid medium for the rapid detection of multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis.

文献详情

资源类型：

Pubmed体系：

作者：

机构： [1]Department of Clinical Laboratory, the First Affiliated Hospital of Nanchang University,Nanchang, Jiangxi, China. [2]Department of Clinical Laboratory, First Teaching Hospital of Tianjin University of TCM,Tianjin, China. [3]Province Tuberculosis Reference Laboratory, Jiangxi Chest Hospital, Nanchang, Jiangxi, China. [4]Clinical Microbiology Laboratory, Baoshan Branch, Shanghai First People’s Hospital, Shanghai, China. [5]Department of Clinical Laboratory, the Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.

出处：

DOI：

ISSN：

关键词： Mycobacterium tuberculosis NRA Drug susceptibility testing Second-line drugs

摘要：

To perform a multicentre study evaluating the performance of the nitrate reductase assay (NRA) using liquid medium for the detection of multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis and to establish the MICs and critical concentrations of rifampicin, isoniazid, ofloxacin, amikacin, kanamycin and capreomycin. The study was carried out in three phases. Phase I determined the MIC of each drug. Phase II established the critical concentration of each drug. Phase III validated critical concentrations for the six drugs tested by the NRA using liquid medium compared with the agar proportion method or MGIT 960 system at each site. The critical concentrations for the six drugs used in the NRA are as follows: rifampicin, 1 mg/L; isoniazid, 0.2 mg/L; ofloxacin, 2 mg/L; amikacin, 2 mg/L; kanamycin, 5 mg/L; capreomycin, 2.5 mg/L. Phase III: Excellent agreement was obtained for all drugs tested at the majority of sites. The accuracy was 97%-100% for rifampicin, 96.8%-99.2% for isoniazid, 98%-100% for ofloxacin, 96.8%-98.5% for amikacin, 96.4%-99.5% for kanamycin and 96.8%-100% for capreomycin. Results for NRA using liquid medium were obtained in a median time of 7 days. NRA performed in liquid medium offers a rapid, economical and feasible method for detection of M. tuberculosis resistance to first- and second-line drugs in resource-limited settings. Copyright © 2018 Elsevier Ltd. All rights reserved.

基金：

语种：

PubmedID：

中科院(CAS)分区：

出版当年[2018]版：

大类 | 3 区医学

小类 | 3 区免疫学 3 区微生物学 3 区呼吸系统

最新[2025]版：

大类 | 4 区医学

小类 | 4 区免疫学 4 区微生物学 4 区呼吸系统

第一作者：

第一作者机构： [1]Department of Clinical Laboratory, the First Affiliated Hospital of Nanchang University,Nanchang, Jiangxi, China.

共同第一作者：

通讯作者：

通讯机构： [1]Department of Clinical Laboratory, the First Affiliated Hospital of Nanchang University,Nanchang, Jiangxi, China. [*1]Department of Clinical Laboratory, the First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China

推荐引用方式(GB/T 7714)：

APA：

MLA：

Multicentre laboratory validation of the nitrate reductase assay using liquid medium for the rapid detection of multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis.

文献详情

相关文献