详情页 - 云南省第一人民医院机构库

当前位置：首页 > 详情页

The Hypothetical Inclusion Membrane Protein CPSIT_0846 Regulates Mitochondrial-Mediated Host Cell Apoptosis via the ERK/JNK Signaling Pathway(Open Access)

58| 导出 | 链接全文 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE

作者：

机构： [a]Department of Public Health Laboratory Sciences, College of Public Health, University of South China, Hengyang, China [b]Key Laboratory of Hengyang for Health Hazard Factors Inspection and Quarantine, College of Public Health, University of South China, Hengyang, China [c]Department of Infection Control, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China [d]Department of Laboratory Medicine, The Second Affiliated Hospital of University of South China, Hengyang, China [e]Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, China [f]Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China

出处：

DOI：

ISSN：

关键词： apoptosis Chlamydia psittaci CPSIT_0846 ERK/JNK signaling pathway mitochondria

摘要：

Chlamydia psittaci is an important zoonotic factor associated with human and animal atypical pneumonia. Resisting host cell apoptosis is central to sustaining Chlamydia infection in vivo. Chlamydia can secrete inclusion membrane proteins (Incs) that play important roles in their development cycle and pathogenesis. CPSIT_0846 is an Inc protein in C. psittaci identified by our team in previous work. In the current study, we investigated the regulatory role of CPSIT_0846 in HeLa cell apoptosis, and explored potential mechanisms. The results showed that HeLa cells treated with CPSIT_0846 contained fewer apoptotic bodies and exhibited a lower apoptotic rate than untreated cells either with Hoechst 33258 fluorescence staining or flow cytometry with or without induction by staurosporine (STS). CPSIT_0846 could increase the phosphorylation of the extracellular signal-regulated kinases 1/2 (ERK1/2) or stress-activated protein kinases/c-Jun amino-terminal kinases (SAPK/JNK) signaling pathways, and the Bcl-2 associated X protein (Bax)/B cell lymphoma 2 (Bcl-2) ratio, levels of cleaved caspase-3/9 and cleaved Poly-ADP-ribose polymerase (PARP) were significantly up-regulated following inhibition of ERK1/2 or SAPK/JNK pathways with U0126 or SP600125. After carbonyl cyanide 3-chlorophenylhydrazone (CCCP) treatment, the mitochondrial membrane potential (MMP) of cells was significantly decreased in control group, but stable in the CPSIT_0846 treated one, and less cytochrome c (Cyt.c) was released into the cytoplasm. Inhibition of the ERK1/2 or SAPK/JNK pathway significantly decreased the JC-1 red-green fluorescence signal, and promoted Cyt.c discharge into the cytoplasm in HeLa cells treated with CPSIT_0846. In conclusion, CPSIT_0846 can regulate mitochondrial pathway-mediated apoptosis in HeLa cells by activating the ERK/JNK signaling pathway. © Copyright © 2021 Tang, Wu, Chen, Chen, Liu, Li, Bai, Chen and Chen.

基金：

语种：

被引次数：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2021]版：

大类 | 2 区医学

小类 | 3 区免疫学 3 区微生物学

最新[2023]版：

大类 | 2 区医学

小类 | 2 区微生物学 3 区免疫学

JCR分区：

出版当年[2020]版：

Q1 MICROBIOLOGY Q2 IMMUNOLOGY

最新[2023]版：

Q1 MICROBIOLOGY Q2 IMMUNOLOGY

影响因子： 4.6 最新[2023版] 5.1 最新五年平均 5.293 出版当年[2020版] 5.882 出版当年五年平均 4.123 出版前一年[2019版] 6.073 出版后一年[2021版]

第一作者：

第一作者机构： [a]Department of Public Health Laboratory Sciences, College of Public Health, University of South China, Hengyang, China [b]Key Laboratory of Hengyang for Health Hazard Factors Inspection and Quarantine, College of Public Health, University of South China, Hengyang, China [c]Department of Infection Control, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China

通讯作者：

通讯机构： [a]Department of Public Health Laboratory Sciences, College of Public Health, University of South China, Hengyang, China [b]Key Laboratory of Hengyang for Health Hazard Factors Inspection and Quarantine, College of Public Health, University of South China, Hengyang, China

推荐引用方式(GB/T 7714)：

APA：

MLA：

The Hypothetical Inclusion Membrane Protein CPSIT_0846 Regulates Mitochondrial-Mediated Host Cell Apoptosis via the ERK/JNK Signaling Pathway(Open Access)

文献详情

相关文献