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Analysis of microRNA expression profiles in human bronchial epithelial cells infected by Chlamydia psittaci

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机构： [a]Department of public health laboratory sciences, College of Public Health, University of South China, Hengyang, China [b]Key Laboratory of Hengyang for Health Hazard Factors Inspection and Quarantine, Hengyang, China [c]Department of Infection Control, The First People's Hospital of Yunnan Province, Kunming, China [d]Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, China

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关键词： Chlamydia psittaci Gene expression profile Gene regulation microRNA Small RNA-Seq

摘要：

Background: Chlamydia psittaci is a pathogen of birds that can cause zoonotic disease in mammals including pneumonia in humans. MicroRNAs (miRNAs) are a class of small non-coding RNA fragments with a length of about 22 nt, which play an important role in regulating gene expression after transcription. Chlamydia infection can cause changes in host cell miRNA expression, but the potential biological function of miRNAs in C. psittaci infection and pathogenesis is not well understood. Methods: Small RNA sequencing (sRNA-Seq) technology was used to characterise miRNA expression in human bronchial epithelial (HBE) cells after C. psittaci infection, and differentially expressed miRNAs were identified. Candidate target genes for these miRNAs were then functionally annotated by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The sRNA-Seq results were partially validated by quantitative real time polymerase chain reaction (qRT-PCR) and miRNA-target networks were constructed using visualization software. Results: We identified 151 differentially expressed miRNAs (46 known miRNAs and 105 novel miRNAs) in C. psittaci-infected HBE cells, of which 140 were upregulated and 11 were downregulated. Of these, 17 known miRNAs were significantly upregulated and two were downregulated using P < 0.05 and |log2FoldChange|>1.5 as threshold criteria. GO enrichment results showed that the predicted targets of these differentially expressed miRNAs were mainly involved in transcriptional regulation and ATP binding. KEGG pathway analysis suggested that the candidate target genes were involved in several important signaling pathways such as MAPK, ErbB, cGMP-PKG, cAMP, mTOR, GNRH, oxytocin, PI3K-Akt and AMPK, which are primarily related to biological processes such as transcription and signal transduction. The qRT-PCR results for miR-2116–3p, miR-3195, miR-663a, miR-10401–5p, miR-124–3p, miR-184, miR-744–5p and hsa-miR-514b-5p were consistent with the sRNA-Seq data. Conclusions: A large amount of miRNA expression profile data relating to C. psittaci infection was obtained, which provides a useful experimental and theoretical basis for further understanding the pathogenic mechanisms of C. psittaci infection. © 2021 Elsevier Ltd

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出版当年[2021]版：

大类 | 3 区医学

小类 | 3 区微生物学 4 区免疫学

最新[2023]版：

大类 | 3 区医学

小类 | 3 区免疫学 3 区微生物学

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出版当年[2020]版：

Q2 MICROBIOLOGY Q3 IMMUNOLOGY

最新[2023]版：

Q2 MICROBIOLOGY Q3 IMMUNOLOGY

影响因子： 3.3 最新[2023版] 3.6 最新五年平均 3.738 出版当年[2020版] 3.664 出版当年五年平均 2.914 出版前一年[2019版] 3.848 出版后一年[2021版]

第一作者：

第一作者机构： [a]Department of public health laboratory sciences, College of Public Health, University of South China, Hengyang, China [b]Key Laboratory of Hengyang for Health Hazard Factors Inspection and Quarantine, Hengyang, China

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通讯作者：

通讯机构： [a]Department of public health laboratory sciences, College of Public Health, University of South China, Hengyang, China [b]Key Laboratory of Hengyang for Health Hazard Factors Inspection and Quarantine, Hengyang, China [*1]Department of public health laboratory sciences, College of Public Health, University of South China, Hengyang, China

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Analysis of microRNA expression profiles in human bronchial epithelial cells infected by Chlamydia psittaci

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