Abstract：0bjective To investigate the effect of ADMA on macrophage migration inhibitory factor fMIF)expression and tumor
necrosis factor-or(rrNF·ot)and IL一8 secretion in THP．1 monocyte。derived macrophages．Methods THP．1 monocytes were
induced to djferentiate into macrophages by a 24．h incubation with 160 nmol／L PMA．The THP．1 monocyte．derived
macrophages were exposed to different concentrations of ADMA for 24 h，and the changes in MIF mRNA and protein
expressions were analyzed with RT-PCR and Western blotting．respectively．Enzyme．1inked immunosorbent assay was used to
detect the levels of TNF-ot and IL一8 in the supernatant of THP一1一derived maerophages following ADMA treatments． Results
ADMA obviously up-regulated MIF mRNA and protein expressions in THP一1．derived macrophages in a concentrati0n．
dependent manner．Exposure of the cells to 1 5 Ixmol／L ADMA for 24 h showed the most potent effect in up．regulating MIF
mRNA and protein expressions．ADMA treatment also resulted in a dose．dependent increase of the levels of TNF．a and IL-8 in
the culture supernatant of the macrophages，and the peak levels occurred following t}le treatment with 1 5 I~mol／L ADMA．
Conclusion ADMA can up-regulate MIF expression and induce TNF。0【and IL一8 secretion in THP．1 monocyte-derived
macrophages．
基金：
National Natural Science Foundation of China(30840043)
语种：
外文
第一作者：
第一作者机构：[1]Faculty ofPharmacy and Yunnan Key Laboratory ofPharmacologyfor Natural Products，Kunming Medical University,Kunming 650031， China.Department ofGeriatric Medicine，Afiliated Kunhua Hospital ofKunming Medical Universitf,[2]Department ofCardiology,First Afiliated Hospital of Kunming Medical Universitf,
