机构：[1]Department of Gastrointestinal Surgery,First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China昆明医科大学附属第一医院[2]Department of Ultrasound,First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China昆明医科大学附属第一医院[3]Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China昆明医科大学附属第一医院
Aberrant expression of circular RNAs (circRNAs) has been demonstrated to be related to the development of colorectal cancer (CRC), the third most common cancer worldwide. However, the mechanism of the effect of circRNA NOP2/Sun domain family, member 2 (circNSUN2) on the malignant biological behavior of CRC remains unclear. In the present study, the expression of circNSUN2 and microRNA (miR)-181a-5p was detected by RT-qPCR. The expression of Rho-associated coiled-coil-containing protein kinase 2 (ROCK2) was measured by western blotting. Cell proliferation was detected by CCK-8 assay. The cell apoptosis rate was measured by flow cytometry. Cell migration ability was evaluated by Transwell assay. The interactions between circNSUN2, miR-181a-5p and ROCK2 were verified by dual-luciferase reporter assay. The results revealed that circNSUN2 was highly expressed in CRC tissues and cell lines. Knockdown of circNSUN2 inhibited the malignant biological behavior of CRC in vivo and in vitro. Moreover, miR-181a-5p was revealed to be a target gene of circNSUN2, and the expression of ROCK2 was negatively regulated by miR-181a-5p. Knockdown of circNSUN2 inhibited proliferation and migration, and induced apoptosis of CRC cells and suppressed tumor growth by targeting miR-181a-5p to decrease ROCK2 expression. In conclusion, circNSUN2 promoted the progression of CRC by sponging miR-181a-5p to increase the expression of ROCK2.
基金：
Kunming Medical University [2019FE001(-039), 2019FE001(-302)]; Leader Training Program in Medical Subjects of Health and Family Planning Commission of Yunnan Province [D-201657]; Department of Science and Technology of Yunnan Province [201801YH00021]; Union Foundation of Yunnan Provincial Science and Technology Department [2019FE001(-039), 2019FE001(-302)]
第一作者机构：[1]Department of Gastrointestinal Surgery,First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Gastrointestinal Surgery,First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China[3]Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China[*1]Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Wuhua, Kunming, Yunnan 650032, P.R. China[*2]Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Wuhua, Kunming, Yunnan 650032, P.R. China
推荐引用方式(GB/T 7714)：
Chi Junlin,Liu Shuang,Wu Zhizhong,et al.circNSUN2 promotes the malignant biological behavior of colorectal cancer cells via the miR-181a-5p/ROCK2 axis[J].ONCOLOGY REPORTS.2021,46(1):doi:10.3892/or.2021.8093.
APA：
Chi, Junlin,Liu, Shuang,Wu, Zhizhong,Shi, Yanqiang,Shi, Chengmin...&Dong, Xiangqian.(2021).circNSUN2 promotes the malignant biological behavior of colorectal cancer cells via the miR-181a-5p/ROCK2 axis.ONCOLOGY REPORTS,46,(1)
MLA：
Chi, Junlin,et al."circNSUN2 promotes the malignant biological behavior of colorectal cancer cells via the miR-181a-5p/ROCK2 axis".ONCOLOGY REPORTS 46..1(2021)
