机构：[1]The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China云南省第一人民医院[2]Institute of Laboratory Medicine, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, China医技片检验科云南省第一人民医院[3]Central Sterile Supply Department, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, China云南省第一人民医院[4]Center for Clinical Medicine Research (Yunnan Provincial Key Laboratory of Clinical Virology), The First People’s Hospital of Yunnan Province, Kunming, Yunnan, China云南省第一人民医院[5]Institute of Ageing Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang, China
Accurate biomarkers to predict the genesis and progression of pancreatic adenocarcinoma (PAAD) are needed in the fight against this deadly disease. Here, we combined multiple datasets (GEO, TCGA and GTEx) to conduct a comprehensive analysis of pancreatic cancer. Through an in-depth analysis, we discovered that the expression of the gene encoding interferon alpha-inducible protein 27 (IFI27) was significantly higher in pancreatic cancer tissues than that in normal tissues, and that higher expression of IFI27 was negatively correlated with the overall survival rate of pancreatic cancer patients. The functional annotation of IFI27 demonstrated relationships to cellular immunity and metabolism, especially glycolysis. Analysis of infiltrating immune cells displayed that higher expression of IFI27 expression correlates with decreased CD8 + T cells and increased M2 macrophages in the tumor immune microenvironment (TIME), then biochemical analyses of a mouse model and immunohistochemical (IHC) staining verified that glycolytic enzymes and M2 macrophages increased significantly in pancreatic cancer tissues. We speculate that IFI27 may affect the tumor microenvironment (TME) of PAAD by regulating cellular immunity and metabolism, thereby promoting the progression of pancreatic carcinoma and worsening the prognosis. These findings of our present study are solid evidence that IFI27 is a potential prognostic biomarker of pancreatic cancer and that it affects the tumor immune microenvironment.
基金：
This work was supported by National Natural Science Foundation of China, grant number No. 82060028.
第一作者机构：[1]The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China[2]Institute of Laboratory Medicine, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, China
通讯作者：
通讯机构：[1]The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China[2]Institute of Laboratory Medicine, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, China[*1]Institute of Laboratory Medicine, the First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China
推荐引用方式(GB/T 7714)：
Huang Shu,Zhao Jinglin,Song Jianxin,et al.Interferon alpha-inducible protein 27 (IFI27) is a prognostic marker for pancreatic cancer based on comprehensive bioinformatics analysis.[J].BIOENGINEERED.2021,12(1):8515-8528.doi:10.1080/21655979.2021.1985858.
APA：
Huang Shu,Zhao Jinglin,Song Jianxin,Li Yanqiong,Zuo Rongxia...&OuYang Hongmei.(2021).Interferon alpha-inducible protein 27 (IFI27) is a prognostic marker for pancreatic cancer based on comprehensive bioinformatics analysis..BIOENGINEERED,12,(1)
MLA：
Huang Shu,et al."Interferon alpha-inducible protein 27 (IFI27) is a prognostic marker for pancreatic cancer based on comprehensive bioinformatics analysis.".BIOENGINEERED 12..1(2021):8515-8528
