Purpose: To study the expression and biological function of microRNA 214 (miR-214) in pancreatic cancer. Methods: 101 patients with pancreatic cancer who came from First People's Hospital of Yunnan Province from December 2013 to December 2016 were selected. 101 pancreatic cancer tissues and 101 adjacent tissues were resected and collected. The miR-214 expression was detected by qRT-PCR. Then the pancreatic cancer cell line AsPC-1 and SW1990 were transfected. MTT assay was used to detect cell viability and flow cytometry was used to detect apoptosis. Transwell chamber assay was used to detect the invasion and migration of cells in vitro. The protein expressions of ING4 in AsPC-1 and SW1990 cells were detected by Western blot. Results: The relative expression of miR-214 in pancreatic cancer was significantly higher than that in adjacent tissues (p<0.05). There was a statistically significant difference between the expression level of miR-214 and T stage of pancreatic cancer (p<0.05). The relative expression of ING4 protein in SW1990 cells of miR-214 mimics group was significantly lower than that of miR-214 control mimics group (p<0.05), and that in AsPC-1 cells of the miR-214 inhibitors group was significantly higher than that in the miR-214 control inhibitors group (p<0.05). Conclusion: In conclusion, the expression of miR-214 is highly expressed in pancreatic cancer tissues, and the down-regulation of ING4 protein expression can inhibit the proliferation, invasion and migration of pancreatic cancer cells, promote their apoptosis, and can be used as a new molecular target for the diagnosis and treatment of pancreatic cancer.