机构：[1]Faculty of Environmental Science and Engineering , Kunming University of Science and Technology , Kunming , People's Republic of China.[2]Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Medicine , Kunming University of Science and Technology , Kunming , People's Republic of China.[3]State Key Laboratory of Pathogen and Biosecurity , Beijing Institute of Microbiology and Epidemiology , Beijing , People's Republic of China.[4]Key Laboratory of Animal Models and Human Disease Mechanisms , Kunming Institute of Zoology, Chinese Academy of Sciences , Kunming , People's Republic of China.[5]Center of Tree Shrew Germplasm Resources , Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College , Kunming , People's Republic of China.[6]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease , First Affiliated Hospital of Guagnzhou Medical University , Guangzhou , People's Republic of China.
Zika virus (ZIKV) is a mosquito-borne flavivirus that caused the public health emergency. Recently, we have proved a novel small animal tree shrew was susceptive to ZIKV infection and presented the most common rash symptoms as ZIKV patients. Here we further cultured the primary cells from different tissues of this animal to determine the tissue tropism of ZIKV infection in vitro. The results showed that the primary cells from tree shrew kidney, lung, liver, skin and aorta were permissive to ZIKV infection and could support viral replication by the detection of viral specific RNA intra- and extra-cells. In comparing, the skin fibroblast and vascular endothelial cells were highly permissive to ZIKV infection with high releasing of active virus particles in supernatants proved by its infectivity in established neonatal mouse model. The expressions of ZIKV envelop and nonstructural protein-1, and the effects and strong immune response of primary tree shrew cells were also detected followed by ZIKV infection. These findings provide powerful in vitro cell-level evidence to support tree shrew as animal model of ZIKV infection and may help to explain the rash manifestations in vivo.
基金：
National Natural Science
Foundation of China (NSFC) under grant No.U1702282
and No. 81471937; the Yunnan provincial Innovative
Team Project under grant No.2015HC030; the Excellent
Young Scientist Program under grant No. 81522025; the
Innovative Research Group of the NSFC under grant No.
81621005 and the Newton Advanced Fellowship from the
UK Academy of Medical Sciences under grant No.
NAF003\1003.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|2 区医学
小类|2 区免疫学2 区微生物学
最新[2025]版：
大类|1 区医学
小类|1 区传染病学1 区微生物学2 区免疫学
第一作者：
第一作者机构：[1]Faculty of Environmental Science and Engineering , Kunming University of Science and Technology , Kunming , People's Republic of China.[2]Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Medicine , Kunming University of Science and Technology , Kunming , People's Republic of China.
通讯作者：
通讯机构：[2]Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Medicine , Kunming University of Science and Technology , Kunming , People's Republic of China.[3]State Key Laboratory of Pathogen and Biosecurity , Beijing Institute of Microbiology and Epidemiology , Beijing , People's Republic of China.
推荐引用方式(GB/T 7714)：
Zhang Li,Shen Zhi-Li,Feng Yue,et al.Infectivity of Zika virus on primary cells support tree shrew as animal model[J].Emerging microbes & infections.2019,8(1):232-241.doi:10.1080/22221751.2018.1559707.
APA：
Zhang Li,Shen Zhi-Li,Feng Yue,Li Dao-Qun,Zhang Na-Na...&Xia Xue-Shan.(2019).Infectivity of Zika virus on primary cells support tree shrew as animal model.Emerging microbes & infections,8,(1)
MLA：
Zhang Li,et al."Infectivity of Zika virus on primary cells support tree shrew as animal model".Emerging microbes & infections 8..1(2019):232-241
医学