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Vitexin 6, a novel lignan, induces autophagy and apoptosis by activating the Jun N-terminal kinase pathway

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机构: [1]Zhejiang Univ, Sch Med, Dept Surg, Womens Hosp, Hangzhou 310006, Zhejiang, Peoples R China [2]Inst Canc Res, Key Lab Mol Biol Med Sci Zhejiang Prov, China Natl Minist Educ, Key Lab Canc Prevent & Intervent, Hangzhou, Zhejiang, Peoples R China [3]First People Hosp Qujing, Dept Oncol, Qujing, Yunnan, Peoples R China [4]Nanjing Med Univ, Affiliated Hosp 1, Nanjing, Jiangsu, Peoples R China [5]Feinstein Inst Med Res, Dept Radiat Med, New Hyde Pk, NY USA
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关键词: apoptosis autophagy Jun N-terminal kinase pathway lignan vitexin

摘要:
Previous studies have reported that vitexins induce cytotoxic effects. In the present study, we investigate a new native lignan vitexin 6 (VB6) in vitro to determine the molecular mechanism underlying its cytotoxicity. We screened and cultured several tumor cell lines and subsequently analyzed VB6 cytotoxicity against 14 different tumor cell lines using a 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The expression of proteins that regulate apoptosis and autophagy was determined using western blot analysis. VB6 showed an excellent cytotoxic effect against various cancer cell lines in vitro. It induced apoptosis and autophagy of cancer cells. VB6-induced apoptosis showed a time-dependent and concentration-dependent relationship with cleaved poly (ADP-ribose) polymerase, cleaved caspase-3, Bax upregulation, and Bcl-2 downregulation. The levels of Beclin-1 and LC3-II, which are markers for cell autophagy, gradually increased after VB6 treatment. Jun N-terminal kinase (JNK) phosphorylation was increased after VB6 treatment, accompanied by upregulation of P-Bcl-2 and P-C-Jun expression. Cotreatment with a JNK inhibitor significantly decreased VB6-induced cell death and downregulated P-Bcl-2, and cleaved PARP and Beclin-1 expression. The new native lignan VB6 inhibits cancer cell proliferation by activating the JNK pathway. We believe that VB6 could be a valuable chemotherapeutic drug after further evaluation.

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出版当年[2013]版:
大类 | 3 区 医学
小类 | 4 区 肿瘤学 4 区 药学
最新[2023]版:
大类 | 4 区 医学
小类 | 4 区 肿瘤学 4 区 药学
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出版当年[2012]版:
Q2 PHARMACOLOGY & PHARMACY Q3 ONCOLOGY
最新[2023]版:
Q3 ONCOLOGY Q3 PHARMACOLOGY & PHARMACY

影响因子: 最新[2023版] 最新五年平均 出版当年[2012版] 出版当年五年平均 出版前一年[2011版] 出版后一年[2013版]

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第一作者机构: [1]Zhejiang Univ, Sch Med, Dept Surg, Womens Hosp, Hangzhou 310006, Zhejiang, Peoples R China [2]Inst Canc Res, Key Lab Mol Biol Med Sci Zhejiang Prov, China Natl Minist Educ, Key Lab Canc Prevent & Intervent, Hangzhou, Zhejiang, Peoples R China
通讯作者:
通讯机构: [1]Zhejiang Univ, Sch Med, Dept Surg, Womens Hosp, Hangzhou 310006, Zhejiang, Peoples R China [*1]Department of Surgery, Women’s Hospital School of Medicine, Zhejiang University, 1, Xueshi Road, Hangzhou, Zhejiang 310006, China
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