机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China[2]Molecular Medicine Center of Yunnan Province, Kunming, Yunnan, China[3]Department of Clinical Laboratory, First People’s Hospital of Yunnan Province, Kunming, Yunnan, China医技片检验科云南省第一人民医院[4]Yunnan SciSpark Biotechnology Co. Ltd., Kunming, Yunnan, China
Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, the rapid and accurate detection of K. pneumoniae is important for the timely treatment of infectious patients. This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse blood with K. pneumoniae were used for sensitivity analysis. The detection limit of the LAMP was 1 bacterium/ reaction. The results showed that the LAMP targeted to ureR_1 is a fast, specific, sensitive, inexpensive, and suitable method for the detection of K. pneumoniae.
基金：
This work was supported by grants from Yunnan
Science and Technology Commission (2015BC001 and
2015DH010).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|4 区医学
小类|4 区生物学4 区医学：研究与实验
最新[2023]版：
大类|4 区医学
小类|4 区生物学4 区医学：研究与实验
JCR分区：
出版当年[2018]版：
Q2BIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q2BIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTALQ3BIOLOGY
第一作者机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China[2]Molecular Medicine Center of Yunnan Province, Kunming, Yunnan, China
共同第一作者：
通讯作者：
通讯机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China[2]Molecular Medicine Center of Yunnan Province, Kunming, Yunnan, China[4]Yunnan SciSpark Biotechnology Co. Ltd., Kunming, Yunnan, China
推荐引用方式(GB/T 7714)：
Li Chao,Fu Gongyu,Shi Yaoqiang,et al.Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method[J].BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH.2019,52(3):doi:10.1590/1414-431X20198186.
APA：
Li, Chao,Fu, Gongyu,Shi, Yaoqiang,Zhang, A-Mei,Xia, Xueshan...&Yang, Guangying.(2019).Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method.BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH,52,(3)
MLA：
Li, Chao,et al."Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method".BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH 52..3(2019)
