机构：[1]Department of Orthopedics, The First People’s Hospital of Yunnan Province, Yunnan, China外科片骨科云南省第一人民医院[2]Department of Plastic Surgery, Central Hospital of Wuhan, Huazhong University of Science and Technology, Wuhan, Hubei, China
Osteoarthritis (OA) is a type of common degenerative joint disorder, in which adipose mesenchymal stem cells (ADSCs) and the secreted exosomes play an important role. The purpose of this study was to investigate the role and mechanism of exosomes derived from ADSCs (ADSC-exos) in OA. The gradient of IL-1 beta concentration was designed to construct the articular chondrocyte model of arthritic mice. The expression of miR-93-5p and ADAMTS9 in articular chondrocytes was detected by reverse transcription quantitative polymerase chain reaction. Dual luciferase reporter gene assay was performed to verify the interaction between them. Monodansylcadaverine staining was used to visualize the autophagosome formation and cell apoptosis was analyzed by flow cytometry. ADSC-exos were authenticated by transmission electron microscope and western blot assay. miR-93-5p was found to be downregulated in IL-1 beta-treated articular chondrocytes compared with OA cartilage while ADAMTS9 was upregulated, which was identified as a direct target gene of miR-93-5p. Silencing of ADAMTS9 attenuated the effects of miR-93-5p. Exosomal miR-93-5p can reduce the release of inflammatory factors in mouse arthritis cell models. This study first described the mechanism under that ADSC-exos inhibited inflammation and alleviated OA through the innovative targets miR-93-5p/ADAMTS9 signal axis. This provided a new method for the treatment of OA.
基金：
Yunnan Basic Research Projects [202101AT070228]; First People's Hospital of Yunnan Province Research Projects [KHBS-2020-002]
第一作者机构：[1]Department of Orthopedics, The First People’s Hospital of Yunnan Province, Yunnan, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Orthopedics, The First People’s Hospital of Yunnan Province, Yunnan, China[*1]Department of Orthopedics, The First People’s Hospital of Yunnan Province, No. 157 Jinbi Road, Kunming 650032, Yunnan, China
推荐引用方式(GB/T 7714)：
Li Yachen,Duan Junjun,Lin Weicheng,et al.Exosomal miR-93-5p regulated the progression of osteoarthritis by targeting ADAMTS9[J].OPEN MEDICINE.2023,18(1):doi:10.1515/med-2023-0668.
APA：
Li, Yachen,Duan, Junjun,Lin, Weicheng&Liu, Jie.(2023).Exosomal miR-93-5p regulated the progression of osteoarthritis by targeting ADAMTS9.OPEN MEDICINE,18,(1)
MLA：
Li, Yachen,et al."Exosomal miR-93-5p regulated the progression of osteoarthritis by targeting ADAMTS9".OPEN MEDICINE 18..1(2023)
