机构：[a]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China[b]Department of Gastroenterology, The First people's Hospital of Yunnan Province, Kunming, Yunnan 650000, China内科片消化内科云南省第一人民医院[c]Departments of Obstetrics and Gynecology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China昆明医科大学附属第一医院[d]Department of Infectious Disease, Yunnan Provincial Key Laboratory of Clinical Virology, The First People's Hospital of Yunnan Province, Kunming, Yunnan 650000,China云南省第一人民医院[e]Research Institute of Nutrition and Food Science, Kunming Medical University, Kunming, Yunnan, 650500, China
Diversity and complexity of Hepatitis B Virus (HBV) may be related to clinical outcome, disease prognosis, and
response to antiviral treatment in infected patients. HBV has been classified into ten genotypes (A-I) and over 50
subgenotypes. However, there are still some variants of HBV that need to be classified. Here, we investigated
genotypic profiles of HBV among 150 patients with chronic hepatitis B in Yunnan, China, and characterized a
novel HBV subgenotype B10. Multiple subgenotypes were identified in 146 subjects with successful sequencing
for the S gene, including genotype B2 (48.6%, 71/146), C1 (34.2%, 50/146), B4 (8.9%, 13/146), C2 (0.7%, 1/
146), C5 (0.7%, 1/146) and an unclassified group (6.8%,10/146). To characterize the unclassified group, seven
HBV complete genomes were successfully amplified and analyzed. The seven strains constituted a potentially
novel B subgenotype that we designated as B10 based on the characteristics of a monophyletic cluster,>4%
genetic distances, no significant evidence of recombination, and no epidemiologic link among individuals.
Moreover, Bayesian analyses showed that HBV B10 originated around the B·C 1.80 thousand years old, suggesting
a much ancient HBV strain. This findings highlighted the importance of continual monitoring of genetic
diversity of HBV strains in Yunnan, China.
基金：
National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China [81560101]; Reserve Talents Project for Young and Middle-Aged Academic and Technical Leaders of Yunnan Province [2019HB012]; Science and Technology Department of Yunnan Province [2018DG010]; Youth Talent Program of Yunnan "Tenthousand Talents Program" [YNWR-QNBJ-2018-054, YNWR-QNBJ2018-169]; Yunnan Applied Basic Research Project [2016FB124]
第一作者机构：[a]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China
共同第一作者：
通讯作者：
通讯机构：[a]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China[d]Department of Infectious Disease, Yunnan Provincial Key Laboratory of Clinical Virology, The First People's Hospital of Yunnan Province, Kunming, Yunnan 650000,China[e]Research Institute of Nutrition and Food Science, Kunming Medical University, Kunming, Yunnan, 650500, China[*1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500,China[*2]Department of Infectious Disease, Yunnan Provincial Key Laboratory of Clinical Virology, The First People's Hospital of Yunnan Province, Kunming, Yunnan 650000, China[*3]Research Institute of Nutrition and Food Science, Kunming Medical University, Kunming, Yunnan, China
推荐引用方式(GB/T 7714)：
Ying Liu,Yue Feng,Yaling Li,et al.Characterization of a novel hepatitis B virus subgenotype B10 among chronic hepatitis B patients in Yunnan, China[J].INFECTION GENETICS AND EVOLUTION.2020,83:doi:10.1016/j.meegid.2020.104322.
APA：
Ying Liu,Yue Feng,Yaling Li,Jing Ma,Yuanyuan Jia...&Yue-Mei Feng.(2020).Characterization of a novel hepatitis B virus subgenotype B10 among chronic hepatitis B patients in Yunnan, China.INFECTION GENETICS AND EVOLUTION,83,
MLA：
Ying Liu,et al."Characterization of a novel hepatitis B virus subgenotype B10 among chronic hepatitis B patients in Yunnan, China".INFECTION GENETICS AND EVOLUTION 83.(2020)
