机构：[1]The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan Province, China云南省第一人民医院[2]The First People’s Hospital of Yunnan Province, Kunming, Yunnan Province, China云南省第一人民医院[3]Yunnan Institute of Digestive Disease, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China昆明医科大学附属第一医院[4]Medical Faculty of Kunming University of Science and Technology, Kunming, Yunnan Province, China
HIV genotyping has led to conflicting results between laboratories. Therefore, identifying the most accurate gene combinations to sequence remains a priority. Datasets of Chinese HIV subtypes based on several markers and deposited in PubMed, Metstr, CNKI, and VIP databases between 2000 and 2015 were studied. In total, 9177 cases of amplification-positive samples from 26 provinces of China were collected and used to classify HIV subtypes based on eight individual genes or a combination thereof. CRF01_ AE, CRF07_ BC, CRF08_ BC and B were the prevalent HIV subtypes in China, accounting for 84.07% of all genotypes. Gag/Env sequencing classified a greater number of HIV subtypes compared to other genes or combination of gene fragments. The geographical distribution of Gag and Gag/Env genotypes was similar to that observed with all genetic markers. Further principal component analysis showed a significantly different geographical distribution pattern of HIV in China for HIV genotypes detected with Gag/Env, which was in line with the distribution of all HIV genotypes in China. Gag/Env sequences had the highest diversity of the eight markers studied, followed by Gag and Gag/Pol/Env; Pol/Env polymorphisms were the least divergent. Gag/Env can serve as a high-resolution marker for HIV genotyping.
基金：
Foundation for Science and Technology Planning Project of Yunnan Provincial Bureau of Health [2012WS63]; Key Joint Funds of the Natural Science Foundation of Yunnan Province; Kunming Medical University [2014FB021]; Yunnan Institute of Digestive Disease Institute [2014NS121]; National Science Foundation of ChinaNational Natural Science Foundation of China [81360069]; Academician Workstation of Yunnan Province; Foundation for Innovative Group of the Gastrointestinal Surgery of Yunnan Province [2012HC013]; Foundation of Medical Leading Talent of Yunnan Province [L-201205]
第一作者机构：[1]The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan Province, China[2]The First People’s Hospital of Yunnan Province, Kunming, Yunnan Province, China[4]Medical Faculty of Kunming University of Science and Technology, Kunming, Yunnan Province, China
共同第一作者：
通讯作者：
通讯机构：[1]The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan Province, China[3]Yunnan Institute of Digestive Disease, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China
推荐引用方式(GB/T 7714)：
Ren L.,Wang H. W.,Xu Y.,et al.Sequencing of Gag/Env association with HIV genotyping resolution and HIV-related epidemiologic studies of HIV in China[J].GENETICS AND MOLECULAR RESEARCH.2016,15(4):-.doi:10.4238/gmr15048870.
APA：
Ren, L.,Wang, H. W.,Xu, Y.,Feng, Y.,Zhang, H. F.&Wang, K. H..(2016).Sequencing of Gag/Env association with HIV genotyping resolution and HIV-related epidemiologic studies of HIV in China.GENETICS AND MOLECULAR RESEARCH,15,(4)
MLA：
Ren, L.,et al."Sequencing of Gag/Env association with HIV genotyping resolution and HIV-related epidemiologic studies of HIV in China".GENETICS AND MOLECULAR RESEARCH 15..4(2016):-
