机构：[1]School of Marine Sciences, Ningbo University, Ningbo, P.R. China.[2]State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, P.R. China.[3]Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Jiangnan University, Wuxi, P.R. China.[4]Department of Clinical Laboratory, The First People’s Hospital of Yunnan Province, Kunming, P.R. China.医技片检验科云南省第一人民医院[5]Department of Clinical Laboratory, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, P.R. China.医技片检验科云南省第一人民医院
Salmonella is among the most serious of foodborne pathogens worldwide and distributed widely in the natural environment; in addition, it has caused severe medical problems and foodborne diseases. Bacterial biofilm was the multicellular community of microorganisms that attached to nonbiological and biological surfaces. Phages and their derivatives are ideal candidates for replacing and compensating antibiotic resistance problems in the future. In this study, a virulent phage of KM15 was isolated from pig slaughterhouse sump samples in Kunming, China. It belonged to the Siphoviridae family, and optimal growth temperature was 42 degrees C, the pH of optimal preservation buffer was 6-7, optimal multiplicity of infection was 0.0001, and the genome size was 41,869 bp. The Salmonella paratyphi A and Salmonella paratyphi B have a broad spectrum of antibiotic resistance and were isolated from clinical patients in the First People's Hospital of Yunnan Province; fortunately, most of them can be lysed by phage KM15. Collaboration of phage KM15 and kanamycin sulfate has a better antibiofilm effect than KM15 and kanamycin sulfate alone, in low-concentration bacterial culture; KM15 has better antibiofilm effect than kanamycin sulfate in high-concentration bacterial culture. The data of this study provided a strong evidence of application of phage to reduce the growth of Salmonella biofilm, which was important for public health.
基金：
This research was funded by Yunnan Science and Technology
Commission from Yunnan provincial Science and
Technology Department and Kunming Medical University,
grant number 2018FE001(-115), Health commission of
Yunnan province talent program, grant number H-2017027.
Sponsored by K.C. Wong Magna Fund in Ningbo University.
The authors gratefully acknowledge the financial support
provided by the National Key Research and Development
Program of China (2017YFC1601704), Projects
31522044, 31671909, and 31772034 of the National Natural
Science Foundation of P.R. China and Program of
Jiangsu Key Laboratory of Advanced Food Manufacturing
Equipment and Technology (FMZ201904), National Firstclass
Discipline Program of Food Science and Technology
( JUFSTR20180205).
第一作者机构：[1]School of Marine Sciences, Ningbo University, Ningbo, P.R. China.[2]State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, P.R. China.[3]Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Jiangnan University, Wuxi, P.R. China.[*1]School of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang 315211, P.R. China
共同第一作者：
通讯作者：
通讯机构：[1]School of Marine Sciences, Ningbo University, Ningbo, P.R. China.[2]State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, P.R. China.[3]Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Jiangnan University, Wuxi, P.R. China.[*1]School of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang 315211, P.R. China
推荐引用方式(GB/T 7714)：
Jiang Liming,Zheng Rui.Isolation and Characterization of a Lytic Salmonella paratyphi Phage and Its Antibiofilm Activity Individually or Collaborative with Kanamycin Sulfate[J].VIRAL IMMUNOLOGY.2020,33(7):521-529.doi:10.1089/vim.2020.0030.
APA：
Jiang, Liming&Zheng, Rui.(2020).Isolation and Characterization of a Lytic Salmonella paratyphi Phage and Its Antibiofilm Activity Individually or Collaborative with Kanamycin Sulfate.VIRAL IMMUNOLOGY,33,(7)
MLA：
Jiang, Liming,et al."Isolation and Characterization of a Lytic Salmonella paratyphi Phage and Its Antibiofilm Activity Individually or Collaborative with Kanamycin Sulfate".VIRAL IMMUNOLOGY 33..7(2020):521-529
