机构：[1]Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, China[2]Medical Faculty, Kunming University of Science and Technology, Kunming, China[3]Department of Science and Education Section, the seventh People’s Hospital of Hangzhou, Hangzhou, China[4]The first People’s Hospital of Yunnan Province, Kunming, China云南省第一人民医院
Background & Aims: Acetaminophen (APAP) is widely used as an antipyretic agent which is safe at therapeutic doses. However, overdose of APAP induces fatal and non-fatal hepatic necroses. The chemical reactive metabolites of APAP initiate toxicity and inflammatory response within the liver and lead to acute liver failure. However, the mechanism underlying APAP-induced liver injury is unknown. Thioredoxin-1 (TRX-1) is an important redox regulator, which plays roles in resisting oxidative stress, regulating inflammation and inhibiting apoptosis. Panaxatriol saponin (PTS) is one of the biologically active fractions of Panax notoginseng which is a traditional Chinese medicine. The aim of this study was to investigate the mechanism on PTS protecting liver from APAP hepatotoxicity. Methods: Mice were divided into three groups, control group, APAP group and APAP combined with PTS group. Alanine aminotransferase (ALT) and tumour necrosis factor-alpha (TNF-alpha) were detected by ELISA. TRX-1 and pro-caspase-12 were examined by Western blotting. Results: Our results showed PTS inhibited the levels of ALT and TNF-alpha by APAP. Pretreatment with PTS ameliorated liver injury induced by APAP. The decrease in TRX-1 expression was restored by PTS, as well as decreased pro-caspase-12 expression was inhibited by PTS. These data suggest that PTS has roles in suppressing the hepatotoxicity by APAP. Conclusion: Panaxatriol saponin ameliorated liver injury by APAP through restoring the expression TRX-1 and inhibiting pro-caspase-12 decrease.
基金：
National Natural Science Foundation of China (No.
81160162), Key Projects in the National Science & Technology
Pillar Program during the Twelfth Five-year Plan
Period (No. 2012ZX10001003-003-019), and a grant from the Key Laboratory of Medical Neurobiology,
Kunming University of Science and Technology.
第一作者机构：[1]Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, China[3]Department of Science and Education Section, the seventh People’s Hospital of Hangzhou, Hangzhou, China
共同第一作者：
通讯作者：
通讯机构：[2]Medical Faculty, Kunming University of Science and Technology, Kunming, China[*1]Medical Faculty, Kunming University of Science and Technology, Kunming 650500, China
推荐引用方式(GB/T 7714)：
Wang Shengdong,Wang Xiao,Luo Fucheng,et al.Panaxatriol saponin ameliorated liver injury by acetaminophen via restoring thioredoxin-1 and pro-caspase-12[J].LIVER INTERNATIONAL.2014,34(7):1068-1073.doi:10.1111/liv.12329.
APA：
Wang, Shengdong,Wang, Xiao,Luo, Fucheng,Tang, Xiaodan,Li, Kui...&Bai, Jie.(2014).Panaxatriol saponin ameliorated liver injury by acetaminophen via restoring thioredoxin-1 and pro-caspase-12.LIVER INTERNATIONAL,34,(7)
MLA：
Wang, Shengdong,et al."Panaxatriol saponin ameliorated liver injury by acetaminophen via restoring thioredoxin-1 and pro-caspase-12".LIVER INTERNATIONAL 34..7(2014):1068-1073
医学